LOCUS       sequence-109867-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression in mammalian cells (production of retrovirus vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Maejima I, Takahashi A, Omori H, Kimura T, Takabatake Y, Saitoh T, 
            Yamamoto A, Hamasaki M, Noda T, Isaka Y, Yoshimori T
  TITLE     Autophagy sequesters damaged lysosomes to control lysosomal 
            biogenesis and kidney injury.
  JOURNAL   EMBO J. 2013 Aug 28;32(17):2336-47. doi: 10.1038/emboj.2013.171. 
            Epub 2013 Aug 6.
  PUBMED    23921551
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2013.171"; journalName: "EMBO J"; date: "2013-08-28- 
            28"; volume: "32"; issue: "17"; pages: "2336-47"
COMMENT     Sequence Label: pMRX-IP-SECFP-Galectin3
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     309..325
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      409..418
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(415..417,418..420,421..879)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITA"
     primer_bind     complement(460..481)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(721..740)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tgagatctta tatggggcac ccccgcccct tgtaaacttc cctgaaccct gacatgacaa
       61 gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg
      121 aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc
      181 acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac
      241 ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg
      301 gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat
      361 cctctagact gccggatccc agtgtggtgg tacgggaatt cctgcagggc caccatggtg
      421 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      481 gtaaacggcc acaggttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      541 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      601 accaccctga cctggggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      661 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgtaccat cttcttcaag
      721 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      781 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      841 gagtacaact acatcagcca caacgtctat atcaccgccg acaagcagaa gaacggcatc
      901 aaggcc
//
