LOCUS       sequence-109895-        5011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E. coli reporter vector with MCS for inserting bacterial promoter 
            region through 5' end of gene of interest, upstream and in-frame 
            with mCherry reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Cress BF, Toparlak OD, Guleria S, Lebovich M, Stieglitz JT, 
            Englaender JA, Jones JA, Linhardt RJ, Koffas MA
  TITLE     CRISPathBrick: Modular Combinatorial Assembly of Type II-A CRISPR 
            Arrays for dCas9-Mediated Multiplex Transcriptional Repression in E.
            coli.
  JOURNAL   ACS Synth Biol. 2015 Mar 30.
  PUBMED    25822415
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Mar 30."
COMMENT     Sequence Label: pCRISPReporter-mCherry
FEATURES             Location/Qualifiers
     source          1..5011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1107..1562
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1194..1213)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1404..1425
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(1677..2468,2469..2537))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2300..2319
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2538..2630)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2711..3299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3200..3219
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3453..3470
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3485..3627
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3553..3572)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3713..3735
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3729..3920)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gggcccgctg ggagttcgta gacggaccta ggtctagatt cagccaaaaa acttaagacc
       61 gccggtcttg tccactacct tgcagtaatg cggtggacag gatcggcggt tttcttttct
      121 cttctcaaca ttactcgcat ccattctcgg cgatcgcgcg gccgctttaa ttaagaattc
      181 aagcttagat ctcaattgct cgagggcggt ggcggtagtg gcggtggcgg ttccggcggt
      241 ggcggttcac atatggtttc aaaaggcgaa gaagacaaca tggcgattat caaggaattt
      301 atgcgtttca aggtccacat ggaaggcagc gtcaatggtc acgaatttga aattgaaggc
      361 gaaggtgaag gccgtccgta tgaaggcacc cagacggcaa aactgaaggt caccaaaggc
      421 ggtccgctgc cgtttgcttg ggatattctg tcaccgcaat tcatgtatgg ttcgaaagcg
      481 tacgttaagc atccggccga tatcccggac tatctgaaac tgtcctttcc ggaaggcttc
      541 aaatgggaac gtgttatgaa cttcgaagat ggcggtgtgg ttaccgtcac gcaggatagc
      601 tctctgcaag acggtgaatt tatttataaa gtgaagctgc gcggcaccaa tttcccgagc
      661 gatggtccgg ttatgcagaa aaagacgatg ggctgggaag cgagttccga acgtatgtac
      721 ccggaagacg gtgccctgaa aggcgaaatc aagcagcgcc tgaaactgaa ggatggcggt
      781 cactatgacg cagaagtgaa aaccacgtac aaggctaaaa agccggtcca actgccgggt
      841 gcatacaacg tgaacatcaa gctggatatc accagccata acgaagacta tacgatcgtt
      901 gaacagtacg aacgtgcaga aggccgccac tctaccggcg gtatggatga actgtacaaa
      961 taaggtaccg gaaacacaga aaaaagcccg cacctgacag tgcgggcttt ttttttcgac
     1021 caaaggaggc tgtctcgtct cgtctcacta gtgctagcaa caaacgcaga atccaagcgt
     1081 cgactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     1141 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     1201 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     1261 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     1321 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     1381 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     1441 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     1501 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     1561 ttctggcggc acgatggcat gagattatca aaaaggatct tcacctagat ccttttaaat
     1621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1741 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1801 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1861 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1981 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2041 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2101 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2161 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2221 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2281 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2341 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2401 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2461 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2521 aaatgttgaa tactcatact cttccttttt caatcatgat tgaagcattt atcagggtta
     2581 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggtcatga
     2641 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     2701 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2761 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2821 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     2881 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2941 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3001 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3061 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3121 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3181 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3241 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3301 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3361 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3421 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3481 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg
     3541 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     3601 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     3661 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     3721 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc
     3781 tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg
     3841 ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg
     3901 gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta
     3961 atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc
     4021 cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga
     4081 aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt
     4141 agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc
     4201 gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca
     4261 gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa
     4321 ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcta
     4381 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt
     4441 cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc
     4501 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     4561 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa
     4621 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
     4681 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
     4741 gtcttcggta tcgtcgtatc ccactaccga gatgtccgca ccaacgcgca gcccggactc
     4801 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
     4861 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
     4921 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
     4981 cagacgcaga cgcgccgaga cagaacttaa t
//
