LOCUS       sequence-109934-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  TITLE     SGC Toronto Q3 2014
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: 4RFQ
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..32
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             45..65
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggagatatac catgcatcat catcatcatc acagcagcgg cagagaaaac ttgtatttcc
       61 agggccataa gtcaatggaa aatgcagctc cctctcaaga cacagacagt ccactcagtg
      121 cagccagcag ttcaaggaac ttggagccac atggaaaaca gccctccttg agagctgcca
      181 aagagcatgc tatgcctaaa gatttaaaga agatgttaga aaataaagtc atagaaacat
      241 taccaggttt ccagcatgtt aagttatcag tagtgaaaac catcttgttg aaagagaact
      301 tcccttatga gggaggctta aaaatctggg aatgtacctt tgacctcctg gcttatttca
      361 caaaggccaa agtgaaattt gctgggaaaa aagtcttgga tcttggttgt ggatcaggtt
      421 tactaggtat aactgcattc aagggagggt ccaaagaaat tcactttcaa gattataaca
      481 gtatggtgat tgatgaagta accttaccta atgtagtagc taactccact ttggaagatg
      541 aagaaaatga tgtaaatgag ccagatgtga aaagatgcag gaaaccaaaa gtaacacaac
      601 tatataaatg ccgatttttt tctggtgagt ggtctgagtt ttgtaagctt gtactaagta
      661 gtgaaaaact ttttgtaaaa tatgatctca ttctcacctc ag
//
