LOCUS       sequence-109935-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses infrared fluorescent tobacco etch virus protease sensor 
            (iTEV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 857)
  AUTHORS   To TL, Piggott BJ, Makhijani K, Yu D, Jan YN, Shu X
  TITLE     Rationally designed fluorogenic protease reporter visualizes 
            spatiotemporal dynamics of apoptosis in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3338-43. doi: 
            10.1073/pnas.1502857112. Epub 2015 Mar 2.
  PUBMED    25733847
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1502857112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-17- 17"; volume: "112"; issue: "11"; pages: "3338-43"
COMMENT     Sequence Label: pcDNA3.1 iTEV
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(2..49)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(392..412)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgtgatgcc ggcggcgttc acgtactcgt gcagcaccat gtggtcccgg ctgcctccct
       61 cgagcttggt tgctggttcc agttctacga tcaggccgcc gttagacgga cggtgaatgg
      121 tacagtccac acggcgcggc ggagaaccca cggtacaacg cagggtcacc ggagccaggt
      181 gcagcgggcc gttcaggtgc ggcaggatct gcaaaggcag atcgccgccc aggtcacgca
      241 gcggacggcc aacaacagag ttggtgttca ggaactccgc agcgttgata gaagcctgga
      301 tcaccacgtt gtctggctct ttcacagcca gcaggatacc gtgcggctga atggagcccg
      361 ccaggtggat ctgctcgcgt tcacagaatt cactctggaa gtagagattt tcgctctcca
      421 gcgtcgcgat gcggttcgca caagtttccg ccagcgcttc gccagcagcg cgaatgtgga
      481 acggaacgaa gcgcggttcg tagtggtggc aagcgatcag accccacaaa cgaccagaca
      541 ccatcagaga gcaaaccagg gtcgcgccaa cgcccatgtc ctgcatgtat ttctggtgga
      601 ccggggacat agagcgcagg caagacaggg acatatccag atcacgaccg ttcagcgggc
      661 tgatgcgcgg ctgtagcgga accggagtat agttcacatc taccagcagg cgaacacggt
      721 tatgttcgta caggcgacga gcgatctgcg gaatagtaga cgccgggtag cggttaccca
      781 ggaacgcttc caggtccgca cgacgacgtt cggacagaat ttcggcatta ccctcttcat
      841 cgaaacgtac taccatc
//
