LOCUS       sequence-110105-       10064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of GAL1 and GAL10 from S. cerevisiae cloned into pRS316.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Cruz C, Houseley J
  TITLE     Endogenous RNA interference is driven by copy number.
  JOURNAL   Elife. 2014 Feb 11;3:e01581. doi: 10.7554/eLife.01581.
  PUBMED    24520161
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-02-11"; volume: "3:e01581. doi"; pages: " 
            10.7554/eLife.01581"
COMMENT     Sequence Label: pRS316 GAL cluster
FEATURES             Location/Qualifiers
     source          1..10064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(2168..2609)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(2174..2197)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    2492..2609
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     promoter        complement(5261..5279)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5300..5316)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5300..5316)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5313..5335)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5324..5340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5348..5354,5355..5372,5373..5378))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(5531..5548)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5702..6290)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6461..7252,7253..7321))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7084..7103
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7322..7426)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    7463..7966
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     8008..8026
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8064..8086)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8186..8205
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8225..8445
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             8446..9249
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(9378..9833)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9515..9536)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9727..9746
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9963..9985
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9977..9994
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9978..9994
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10001..10020
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10001..10019
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     join(10052..10064,1..4)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(10052..10064,1..4)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gatccgtcta ggaaatcggt gaaagctttt tttttttcct atcttcttgt ccatcggaat
       61 ttttctgtca tttcttttcc tcctcgcgct tgtctactaa aatctgaatt gtccaaattc
      121 agtacaaaat taatcagtag gacaaagggt tctcgtagag tccccggaaa aaaaaaagga
      181 caaaaagttt caagacggca atctcttttt actgcatctc gtcagttggc aacttgccaa
      241 gaacttcgca aatgactttg acatatgata agacgtcaac tgccccacgt acaataacaa
      301 aatggtagtc atatcatgtc aagaataggt atccaaaacg cagcggttga aagcatatca
      361 agaattttgt ccctgtgttt taaagtttgt ggataatcga aatctcttac attgaaaaca
      421 ttatcataca atcatttatt aagtagttga agcatgtatg aactataaaa gtgttactac
      481 tcgttattat tgcgtatttt gtgatgctaa agttatgagt agaaaaaaat gagaagttgt
      541 tctgaacaaa gtaaaaaaaa gaagtatact tataattcat atagacagct gcccaatgct
      601 ggtttagaga cgatgatagc attttctagc tcagcatcag tgatcttagg gtacttgacc
      661 ttgtagaact cattggcaag ggcttctttt accttttcta tgttgccatt tgggccccct
      721 ggaaccaagt gaacagtaca accaccccag ccagctccgg tcaaacggga accatatgat
      781 ccatttgaca aagcaatgga acaaattttg tcaatctctg gacaagaaca ttcgtaaagt
      841 ttatcgcaag aagcttgaga ctcgttcatc aaggcaccaa attgcttgaa aaagtcttcg
      901 tcggcagtaa agctcgctgt agtcattaat ttcacagcct tcaagactct taaagattca
      961 gaatacacat gcttagccct ctgatatagc tttaagactt gaaatctcac tggagatgtt
     1021 gttaagtagt ctcttgtgaa ttcttcgcga gaacaattca aggattgtgc gacatcgtca
     1081 acactaaagc cctgtttctt attggcgaga gactcttcaa ctagtactag catctttgtt
     1141 aaccgttcga tgccggattc aatatcgccg ttccagggtg tggaaatgtt gtgatatctg
     1201 gcataataaa cgttcatgaa atctcttaga ttacctttat tcgtgctcga tccttctttt
     1261 ccagaaagta aaacaacacc gtacgtggca gctaaaacat ttgcagctgt agtgacttct
     1321 accactctta aattatagtt ggttggggcg gtttcaaact tgttagatac aacaagggtg
     1381 ttcgcaataa caaagctaat ttcatggttt tttaattgcg gaaatttaaa cggagtagcc
     1441 ttcaactgcg gtttgaactc aacgtataga gcatgatctt cctcaccgca aacagaggca
     1501 gcctgatcca taccgccatt gttaacacca acataatgtt ctgcaacgac cgtaatacgc
     1561 attaaatttt gcttggacat atgataacca gggcccatat tcgctttaac aacagctaaa
     1621 gcaacggcac aaatgaatgc ggccgaagaa gacaatccac tgccagttgg tacatcaccc
     1681 tcacagaaga cttgcagccc ggccagagga gcactggcaa acctttccgg tgcaagtttc
     1741 tttagaaaag agtgagcaac atggagacca catttaaagt aattagacca gtccgacaca
     1801 gaaggatcaa ttgtgacata agaaccgtcc aacggcaaat cgaacttcct ttgagcaaat
     1861 ttgggatcag catttattaa ggtaatggat ggatttttct cgttcaaaac tttgacggcg
     1921 caaagcatat caaaatcaat agctaaaggt aaaaccgaga agtcacaata atcaatatgt
     1981 tcaccaatta gattgactct accaggcgat ctagcaacaa aatccggttt agcatcataa
     2041 gcgcttataa atttcttaat tatgctcggg cacttttcgg ccaatggtct tggtaattcc
     2101 tttgcgctag aattgaactc aggtacaatc acttcttctg aatgagattt agtcattata
     2161 gttttttctc cttgacgtta aagtatagag gtatattaac aattttttgt tgatactttt
     2221 atgacatttg aataagaagt aatacaaact gaaaatgttg aaagtattag ttaaagtggt
     2281 tatgcagctt ttccatttat atatctgtta atagatcaaa aatcatcgct tcgctgatta
     2341 attaccccag aaataaggct aaaaaactaa tcgcattatc atcctatggt tgttaatttg
     2401 attcgttaat ttgaaggttt gtggggccag gttactgcca atttttcctc ttcataacca
     2461 taaaagctag tattgtagaa tctttattgt tcggagcagt gcggcgcgag gcacatctgc
     2521 gtttcaggaa cgcgaccggt gaagacgagg acgcacggag gagagtcttc cgtcggaggg
     2581 ctgtcgcccg ctcggcggct tctaatccgt acttcaatat agcaatgagc agttaagcgt
     2641 attactgaaa gttccaaaga gaaggttttt ttaggctaag ataatggggc tctttacatt
     2701 tccacaacat ataagtaaga ttagatatgg atatgtatat ggtggtaatg ccatgtaata
     2761 tgattattaa acttctttgc gtccatccaa aaaaaaagta agaatttttg aaaattcaat
     2821 ataaatgaca gctcagttac aaagtgaaag tacttctaaa attgttttgg ttacaggtgg
     2881 tgctggatac attggttcac acactgtggt agagctaatt gagaatggat atgactgtgt
     2941 tgttgctgat aacctgtcga attcaactta tgattctgta gccaggttag aggtcttgac
     3001 caagcatcac attcccttct atgaggttga tttgtgtgac cgaaaaggtc tggaaaaggt
     3061 tttcaaagaa tataaaattg attcggtaat tcactttgct ggtttaaagg ctgtaggtga
     3121 atctacacaa atcccgctga gatactatca caataacatt ttgggaactg tcgttttatt
     3181 agagttaatg caacaataca acgtttccaa atttgttttt tcatcttctg ctactgtcta
     3241 tggtgatgct acgagattcc caaatatgat tcctatccca gaagaatgtc ccttagggcc
     3301 tactaatccg tatggtcata cgaaatacgc cattgagaat atcttgaatg atctttacaa
     3361 tagcgacaaa aaaagttgga agtttgctat cttgcgttat tttaacccaa ttggcgcaca
     3421 tccctctgga ttaatcggag aagatccgct aggtatacca aacaatttgt tgccatatat
     3481 ggctcaagta gctgttggta ggcgcgagaa gctttacatc ttcggagacg attatgattc
     3541 cagagatggt accccgatca gggattatat ccacgtagtt gatctagcaa aaggtcatat
     3601 tgcagccctg caatacctag aggcctacaa tgaaaatgaa ggtttgtgtc gtgagtggaa
     3661 cttgggttcc ggtaaaggtt ctacagtttt tgaagtttat catgcattct gcaaagcttc
     3721 tggtattgat cttccataca aagttacggg cagaagagca ggtgatgttt tgaacttgac
     3781 ggctaaacca gatagggcca aacgcgaact gaaatggcag accgagttgc aggttgaaga
     3841 ctcctgcaag gatttatgga aatggactac tgagaatcct tttggttacc agttaagggg
     3901 tgtcgaggcc agattttccg ctgaagatat gcgttatgac gcaagatttg tgactattgg
     3961 tgccggcacc agatttcaag ccacgtttgc caatttgggc gccagcattg ttgacctgaa
     4021 agtgaacgga caatcagttg ttcttggcta tgaaaatgag gaagggtatt tgaatcctga
     4081 tagtgcttat ataggcgcca cgatcggcag gtatgctaat cgtatttcga agggtaagtt
     4141 tagtttatgc aacaaagact atcagttaac cgttaataac ggcgttaatg cgaatcatag
     4201 tagtatcggt tctttccaca gaaaaagatt tttgggaccc atcattcaaa atccttcaaa
     4261 ggatgttttt accgccgagt acatgctgat agataatgag aaggacaccg aatttccagg
     4321 tgatctattg gtaaccatac agtatactgt gaacgttgcc caaaaaagtt tggaaatggt
     4381 atataaaggt aaattgactg ctggtgaagc gacgccaata aatttaacaa atcatagtta
     4441 tttcaatctg aacaagccat atggagacac tattgagggt acggagatta tggtgcgttc
     4501 aaaaaaatct gttgatgtcg acaaaaacat gattcctacg ggtaatatcg tcgatagaga
     4561 aattgctacc tttaactcta caaagccaac ggtcttaggc cccaaaaatc cccagtttga
     4621 ttgttgtttt gtggtggatg aaaatgctaa gccaagtcaa atcaatactc taaacaatga
     4681 attgacgctt attgtcaagg cttttcatcc cgattccaat attacattag aagttttaag
     4741 tacagagcca acttatcaat tttataccgg tgatttcttg tctgctggtt acgaagcaag
     4801 acaaggtttt gcaattgagc ctggtagata cattgatgct atcaatcaag agaactggaa
     4861 agattgtgta accttgaaaa acggtgaaac ttacgggtcc aagattgtct acagattttc
     4921 ctgatttgcc agcttactat ccttcttgaa aatatgcact ctatatcttt tagttcttaa
     4981 ttgcaacaca tagatttgct gtataacgaa ttttatgcta ttttttaaat ttggagttca
     5041 gtgataaaag tgtcacagcg aatttcctca catgtaggga ccgaattgtt tacaagttct
     5101 ctgtaccacc atggagacat caaaaattga aaatctatgg aaagatatgg acggtagcaa
     5161 caagaatata gcacgagccg cggagttcat ttcgttactt ttgatatcac tcacaactat
     5221 tgcgagcgct cgaggggggg cccggtacca gcttttgttc cctttagtga gggttaattt
     5281 cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     5341 ttccacacaa cataggagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     5401 ggtaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     5461 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5521 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5581 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5641 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5701 ttttccatag gctcggcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5761 ggcgaaaccc gacaggacta taaagatacc aggcgttccc ccctggaagc tccctcgtgc
     5821 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5881 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     5941 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6001 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6061 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6121 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     6181 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6241 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6301 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6361 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6421 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6481 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctgcccgtcg
     6541 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6601 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6661 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6721 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6781 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6841 caaggcgagt tacatgatcc cccatgttgt gaaaaaaagc ggttagctcc ttcggtcctc
     6901 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     6961 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7021 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7081 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7141 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7201 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7261 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7321 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7381 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7441 aagtgccacc tgggtccttt tcatcacgtg ctataaaaat aattataatt taaatttttt
     7501 aatataaata tataaattaa aaatagaaag taaaaaaaga aattaaagaa aaaatagttt
     7561 ttgttttccg aagatgtaaa agactctagg gggatcgcca acaaatacta ccttttatct
     7621 tgctcttcct gctctcaggt attaatgccg aattgtttca tcttgtctgt gtagaagacc
     7681 acacacgaaa atcctgtgat tttacatttt acttatcgtt aatcgaatgt atatctattt
     7741 aatctgcttt tcttgtctaa taaatatata tgtaaagtac gctttttgtt gaaatttttt
     7801 aaacctttgt ttattttttt ttcttcattc cgtaactctt ctaccttctt tatttacttt
     7861 ctaaaatcca aatacaaaac ataaaaataa ataaacacag agtaaattcc caaattattc
     7921 catcattaaa agatacgagg cgcgtgtaag ttacaggcaa gcgatccgtc ctaagaaacc
     7981 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     8041 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     8101 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     8161 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccac
     8221 gcttttcaat tcaattcatc attttttttt tattcttttt tttgatttcg gtttctttga
     8281 aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt
     8341 agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta
     8401 acccaactgc acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca
     8461 tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg
     8521 cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg
     8581 gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg
     8641 actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat
     8701 tttttactct tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac
     8761 tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg
     8821 ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga
     8881 ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact
     8941 aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa
     9001 agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt
     9061 ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga tgtggtctct
     9121 acaggatctg acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag
     9181 gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag
     9241 caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca aattagagct
     9301 tcaatttaat tatatcagtt attaccctgc ggtgtgaaat accgcacaga tgcgtaagga
     9361 gaaaataccg catcaggaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
     9421 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     9481 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     9541 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     9601 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     9661 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     9721 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     9781 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcgcgcca
     9841 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     9901 acgccagctg gcgaaggggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     9961 ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg
    10021 cgaattggag ctccaccgcg gtggcggccg ctctagaact agtg
//
