LOCUS       sequence-110200-        1761 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes 6XHis_3XFlag as a Type 3a part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK040
FEATURES             Location/Qualifiers
     source          1..1761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..85
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             89..106
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(126..160)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(231..890)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     805..824
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1072..1660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1561..1580
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atggattaca aggacgatga tgataaggac tacaaagatg atgatgacaa
       61 agattataag gacgatgatg ataaaggtca tcatcatcat catcacggtt cttgagacca
      121 gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
      181 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
      241 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
      301 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
      361 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
      421 tcaaaactgg tgaaactcac ccagggattg gctgaaacga aaaacatatt ctcaataaac
      481 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
      541 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
      601 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
      661 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
      721 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
      781 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
      841 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
      901 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
      961 tggtgaaagt tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga
     1021 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1081 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1141 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1201 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1261 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1321 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1381 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1441 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1501 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1561 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1621 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1681 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1741 tgattctgtg gataaccgta g
//
