LOCUS       sequence-110219-        2383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes mRuby2 as a Type 4a part to be used in the Dueber YTK 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Lee ME, DeLoache WC, Cervantes B, Dueber JE
  TITLE     A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly
  JOURNAL   ACS Synth Biol. 2015 Apr 14.
  PUBMED    25871405
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 14."
COMMENT     Sequence Label: pYTK059
FEATURES             Location/Qualifiers
     source          1..2383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..721
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     terminator      complement(748..782)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(853..1512)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1427..1446
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1694..2282
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2183..2202
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa tccgtgtcca aaggagagga gttaatcaag gaaaacatga gaatgaaagt
       61 tgtcatggag ggctccgtta atggtcacca attcaagtgt acaggggaag gtgaaggtaa
      121 tccttacatg ggtacacaaa ctatgagaat taaagtaatt gaaggcggac cactaccatt
      181 tgcatttgac attctggcaa cgtcattcat gtacggatca cgaactttca tcaagtaccc
      241 taaaggtata ccagactttt tcaagcaatc ttttccagag ggttttacat gggaaagggt
      301 tacaagatac gaagatgggg gtgtcgtcac agttatgcaa gatacttcat tagaagatgg
      361 ctgccttgtc tatcatgtgc aagtaagagg ggtgaatttt ccttctaacg gacctgtgat
      421 gcagaaaaag accaaaggtt gggaaccaaa tactgaaatg atgtacccag ctgatggagg
      481 tttgagaggc tacacacaca tggcgcttaa agttgatggt ggaggtcatt tgtcttgtag
      541 ttttgttacc acttatcgtt ctaaaaagac tgttggcaat atcaaaatgc caggaataca
      601 tgctgtagac cacagactag aaagactcga agagagcgat aacgaaatgt tcgttgtaca
      661 gagagagcat gccgtagcca aatttgctgg cttaggcggt ggtatggatg aattgtataa
      721 gtaactcgag tggctgagac cagaccaata aaaaacgccc ggcggcaacc gagcgttctg
      781 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag
      841 ctcgatatca aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
      901 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
      961 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     1021 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgaaac
     1081 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     1141 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     1201 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     1261 tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg
     1321 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     1381 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     1441 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     1501 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
     1561 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaatca
     1621 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1681 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1741 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1801 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     1861 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1921 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1981 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2041 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2101 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2161 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2221 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2281 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2341 tgttctttcc tgcgttatcc cctgattctg tggataaccg tag
//
