LOCUS       sequence-110259-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tobacco etch virus protease (TEV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   To TL, Piggott BJ, Makhijani K, Yu D, Jan YN, Shu X
  TITLE     Rationally designed fluorogenic protease reporter visualizes 
            spatiotemporal dynamics of apoptosis in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3338-43. doi: 
            10.1073/pnas.1502857112. Epub 2015 Mar 2.
  PUBMED    25733847
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1502857112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-17- 17"; volume: "112"; issue: "11"; pages: "3338-43"
COMMENT     Sequence Label: pcDNA3.1 TEV (full-length)
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..29)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(45..74)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      807..816
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(842..861)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(843..861)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gtttaaactc aatggtgatg gtgatgatga ccggtatgca tattcagatc ctcttctgag
       61 atgagttttt gttcgaaccg cgggccctct agactcgagt tatcaaagct gagtggcttc
      121 cttaacgggc tggaagggtt cctcgggctt gaccatgaac accttatgtc cgccccaaag
      181 aacgctgtcg gcgttcagcc tccagccgct cacccactgc tgcgcctctt gattggtcaa
      241 cagctccatg aagtttttcg gcacgctcgt gaagtagttg ttggtattgg taaaattgct
      301 ggcgctgtgg atgcccacga tgaagccgtc cctagtgctc accaatgggc tgccgcattg
      361 gccatccttc gtctgaatcc agtgcttcca gaagatgccg tcgctgctgg ggaaggtgca
      421 gctggtatca gagaccatgc tgctcatgga cttggtctgg aagttggtgg tcaccaggca
      481 gattcgctcc tccctttgtg gctccctgaa cttcagtttc tggggaaagg gggggaagtc
      541 cttgggcatc ctgatgataa tcatgtccct gccgtcgatc aggtgctgtt gcagggtggt
      601 tgtgttcttc actttgaaca cgccgtgcag gctttgcacc agcagtgtgc cgttattcct
      661 cctgaacaga tgcttgttgg tgatgatgaa ggggccgaag ccgatgccgt acagactagt
      721 ggtgtgaccg tcgctctcgt tggtcaggtg acagatggtg ctggagatcg ggttgtagtc
      781 cctcgggccc ttgaaaaggc tctcgcccat ggtggcaagc ttaactagcc agcttgggtc
      841 tccctatagt gagtcgtatt aatttcgata agccagtaag ca
//
