LOCUS       sequence-110284-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal eCFP Strep-tag II followed by URA3 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1312
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(91..288)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggccgcatag gccactagtg gatctgatat catcgatgaa ttcgagctcg ttttcgacac
       61 tggatggcgg cgttagtatc gaatcgacag cagtatagcg accagcattc acatacgatt
      121 gacgcatgat attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg
      181 aaaaaaaata taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa
      241 ctatacaaat gacaagttct tgaaaacaag aatcttttta ttgtcagtac agattataat
      301 tggccagtct ttttcaaata agcattccaa ccagcatctc tatacctttt accttcaata
      361 tctggatctc ttcctttacc aaacaatcct ctaccaacaa tgataatatc agttccagtg
      421 ctaacaactt catcaacagt tctatattgt tgtcctaatc catcaccttt atcatctaat
      481 ccaactccag gtgtcataat aagccaatca aatccttctt cttgtccacc catatcacgt
      541 tgggcaataa atccaataac aaattcctta tcggatttag caatttcaac agttttttga
      601 gaatattctc catatgctaa tgatcccact gatgataatt cagctaacat caataaccct
      661 cttggctctt ggttggtggt ggtttcttta gctccctgtt ttaatccttc aactactcca
      721 ttcccagtga caccatgagc attagtaata tctgcccaac tactaatttt ataaactcca
      781 ccaatatatt gtttcttcac ggtattacca atatcagcaa attttctatc ttcaaaaatc
      841 ataaattgat gtttacgtga aagttctaat aatggttcaa tagtggattc ataggaaaaa
      901 tcattgatta tatcaatatg agtcttgatt aagcatacat aaggacccaa tttatcaatt
      961 aattca
//
