LOCUS       sequence-110336-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Sp dCas9-GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-dCas9-GFP
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     46..70
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      163..172
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             175..195
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tatcagtgat agagatctcc ctatcagtga tagagatcgt cgacgagctc gtttagtgaa
       61 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga
      121 ccgatccagc ctaccggtta aggtgaacgc gtgcaggctg gcgccaccat ggcccctaaa
      181 aagaaacgca aggtcgaatt cggaagcgga agcgacaaga agtactccat tgggctcgct
      241 atcggtacca acagcgtcgg ctgggccgtc attacggacg agtacaaggt gccgagcaaa
      301 aaattcaaag ttctgggcaa taccgatcgc cacagcataa agaagaacct cattggagcc
      361 ctcctgttcg actccgggga gacggccgaa gccacgcggc tcaaaagaac agcacggcgc
      421 agatataccc gcagaaagaa tcggatctgc tacctgcagg agatctttag taatgagatg
      481 gctaaggtgg atgactcttt cttccatagg ctggaggagt cctttttggt ggaggaggat
      541 aaaaagcacg agcgccaccc aatctttggc aatatcgtgg acgaggtggc gtaccatgaa
      601 aagtacccaa ccatatatca tctgaggaag aagctggtag acagtactga taaggctgac
      661 ttgcggttga tctatctcgc gctggcgcac atgatcaaat ttcggggaca cttcctcatc
      721 gagggggacc tgaacccaga caacagcgat gtcgacaaac tctttatcca actggttcag
      781 acttacaatc agcttttcga ggagaacccg atcaacgcat ccggcgttga cgccaaagca
      841 atcctgagcg ctaggc
//
