LOCUS       sequence-110343-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of St1 dCas9-3XGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    14..32
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     42..66
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      159..168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             171..191
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agtgatagag atctccctat cagtgataga gatcgtcgac gagctcgttt agtgaaccgt
       61 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
      121 tccagcctac cggttaaggt gaacgcgtgc aggctggcgc caccatggcc cctaaaaaga
      181 aacgcaaggt cgaattcgga agcgacctgg tgctgggcct ggccatcggc atcggcagcg
      241 tgggcgtggg catcctgaac aaggtgaccg gcgagatcat ccacaagaac agtcgcatct
      301 tccctgctgc tcaggctgag aacaacctgg tgcgccgcac caaccgccag ggtcgccggc
      361 ttgctcgccg caagaagcac cggcgcgtgc gcctgaaccg cctgttcgag gagagcggcc
      421 tgatcaccga cttcaccaag atcagcatca acctgaaccc ctaccagctg cgcgtgaagg
      481 gcctgaccga cgagctgagc aacgaggagc tgttcatcgc cctgaagaac atggtgaagc
      541 accgcggcat cagctacctg gacgacgcca gcgacgacgg caacagcagc gtgggcgact
      601 acgcccagat cgtgaaggag aacagcaagc agctggagac caagaccccc ggccagatcc
      661 agctggagcg ctaccagacc tacggccagc tgcgcggcga cttcaccgtg gagaaggacg
      721 gcaagaagca ccgcctgatc aacgtgttcc ccaccagcgc ctaccgcagc gaggccctgc
      781 gcatcctgca gacccagcag gagttcaac
//
