LOCUS       sequence-110355-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under 
            MtBCP1 promoter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMB-TPr
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(9..719)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(287..306)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     556..574
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    721..745
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 taagttgctt acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt
       61 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg
      121 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
      181 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg
      241 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt
      301 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg
      361 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
      421 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
      481 tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag
      541 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
      601 ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc
      661 ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccatt
      721 tccactttgt acaagaaagc tgggtcgtag tctgtggttg ggagctgctc gtgtgtggtg
      781 ttgatgaaga aaacttcgta gatgagtcca gcgattccac cgccgacgag aggtccggcc
      841 cagtagaccc agtggttggt ccatgtccag ctcaccaccg ctggtccgaa agcca
//
