LOCUS       sequence-110356-         598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Creating dVP16AD hemidrivers from MiMIC lines with inserts into 
            coding introns in Phase 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(1)-T2A-dVP16AD-Hsp70
FEATURES             Location/Qualifiers
     source          1..598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..70
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             239..292
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             323..343
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc tccccgggcg cgtactccac
       61 ctcacccatc tggtccatca tgatggtcga ctctagaagt cgatccaaca tggcgacttg
      121 tcccatcccc ggcatgttta aatatactaa ttattcttga actaatttta atcaaccgat
      181 ttatctctct tccgcaggtg gcggaggttc cggtggaagc ggaggtagcg gcggatccga
      241 gggccgcggc agcctgctga cctgcggcga tgtggaggag aaccccgggc ccatggataa
      301 agcggaatta attcccgagc ctccaaaaaa gaagagaaag gtcgaattgg gtaccgcccc
      361 ccccaccgac gtgtccctgg gcgatgagct gcacctggat ggcgaggatg tggccatggc
      421 ccacgccgat gccctggatg acttcgacct ggatatgctg ggcgatggcg acagccccgg
      481 ccccggcttc accccccacg atagcgcccc ctacggcgcc ctggacatgg ccgatttcga
      541 gttcgagcaa atgttcaccg acgccctggg catcgatgag tacggcggcg ctagcgga
//
