LOCUS       sequence-110454-         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of St1 dCas9-3XGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..46)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(53..73)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(80..793)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 atccagaggt tgattatcta gattaaacct tacgcttctt tttcggtgag cccactttcc
       61 gctttttctt tggctcgagt ttgtacagtt catccatacc atgcgtgatg cccgctgcgg
      121 ttacgaactc cagcagaacc atatgatcgc gtttctcgtt cggatcttta gacagaacgc
      181 tttgcgtgct cagatagtga ttgtctggca gcagaacagg accatcaccg attggagtgt
      241 tttgctggta gtgatcagcc agctgcacgc tgccatcctc cacgttgtgg cgaattttaa
      301 aattcgcttt aatgccattt ttttgtttat cggcggtgat gtaaacattg tggctgttaa
      361 aattgtattc cagcttatgg cccaggatat tgccgtcttc tttaaagtca atgcctttca
      421 gctcaatgcg gtttaccagg gtatcgcctt caaatttcac ttccgcacgc gttttgtacg
      481 tgccgtcatc cttaaaggaa atcgtgcgtt cctgcacata gccttccggc atggcggact
      541 tgaagaagtc atgctgcttc atatggtccg gataacgagc aaagcactga acaccataag
      601 tcagcgtcgt taccagagtc ggccaaggta ccggcagttt accagtagta cagatgaact
      661 tcagcgtcag tttaccatta gttgcgtcac cttcaccctc gccacgcacg gaaaacttat
      721 gaccgttgac atcaccatcc agttccacca gaatagggac gacaccagtg aacagctctt
      781 cgcctttacg catgctacca tcgatgctac ctttgtacag ttcatccata ccatgcgtga
      841 tgcccgctgc ggttacgaac tccagcagaa ccatatgatc gcgtttctcg ttcggatctt
      901 tagacagaac gctttgcgtg ctcagatagt gattgtctgg cagcagaaca ggaccatca
//
