LOCUS       sequence-110536-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of St1 dCas9-3XGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..41)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(48..761)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 gaggttgatt atctagatta cactttccgc tttttctttg gctcgagttt gtacagttca
       61 tccataccat gcgtgatgcc cgctgcggtt acgaactcca gcagaaccat atgatcgcgt
      121 ttctcgttcg gatctttaga cagaacgctt tgcgtgctca gatagtgatt gtctggcagc
      181 agaacaggac catcaccgat tggagtgttt tgctggtagt gatcagccag ctgcacgctg
      241 ccatcctcca cgttgtggcg aattttaaaa ttcgctttaa tgccattttt ttgtttatcg
      301 gcggtgatgt aaacattgtg gctgttaaaa ttgtattcca gcttatggcc caggatattg
      361 ccgtcttctt taaagtcaat gcctttcagc tcaatgcggt ttaccagggt atcgccttca
      421 aatttcactt ccgcacgcgt tttgtacgtg ccgtcatcct taaaggaaat cgtgcgttcc
      481 tgcacatagc cttccggcat ggcggacttg aagaagtcat gctgcttcat atggtccgga
      541 taacgagcaa agcactgaac accataagtc agcgtcgtta ccagagtcgg ccaaggtacc
      601 ggcagtttac cagtagtaca gatgaacttc agcgtcagtt taccattagt tgcgtcacct
      661 tcaccctcgc cacgcacgga aaacttatga ccgttgacat caccatccag ttccaccaga
      721 atagggacga caccagtgaa cagctcttcg cctttacgca tgctaccatc gatgctacct
      781 ttgtacagtt catccatacc atgcgtgatg cccgctgcgg ttacgaactc cagcagaacc
      841 atatgatcgc gtttctcgtt cggatcttta gacagaa
//
