LOCUS       sequence-110549-        2872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  receiving vector of terminators for the construction of standard 
            terminator parts in YeastFab work.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HcKan_T
FEATURES             Location/Qualifiers
     source          1..2872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    81..102
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    203..224
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(239..244,245..262,263..269)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    277..293
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             311..322
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             329..1006
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1051..1122
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1138..1165
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    1172..1192
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      1249..1280
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1281..1383
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1384..2199
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1457..1476)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2223..2250
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2262..2849)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2342..2361)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(join(2871..2872,1..28))
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag
       61 tccgtctcgt agcagagacc gaattcgcgg ccgcttctag agcaatacgc aaaccgcctc
      121 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      181 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      241 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      301 catactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt
      361 catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg
      421 tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg
      481 tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc
      541 ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt
      601 caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc
      661 ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc
      721 cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta
      781 cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg
      841 tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg
      901 tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt
      961 tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg
     1021 ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1081 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1141 tcaccttcgg gtgggccttt ctgcgtttat atactagtag cggccgctgc agggtctctc
     1201 ctccgagacg actgaccatt taaatcatac ctgacctcca tagcagaaag tcaaaagcct
     1261 ccgaccggag gcttttgact tgatcggcac gtaagaggtt ccaactttca ccataatgaa
     1321 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
     1381 aaaatgagcc atattcaacg ggaaacgtct tgctcgaggc cgcgattaaa ttccaacatg
     1441 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
     1501 atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
     1561 agcgttgcca atgatgttac agatgagatg gtcaggctaa actggctgac ggaatttatg
     1621 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
     1681 gcgatcccag ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
     1741 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
     1801 ccttttaacg gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
     1861 ttggttggtg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     1921 aaagaaatgc ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc
     1981 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     2041 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     2101 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     2161 aaattgcagt ttcacttgat gctcgatgag tttttctaat gagggcccaa atgtaatcac
     2221 ctggctcacc ttcgggtggg cctttctgcg ttgctggcgt ttttccatag gctccgcccc
     2281 cctgacgagc atcacaaaaa tcgatgctca agtcagaggt ggcgaaaccc gacaggacta
     2341 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2401 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2461 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2521 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2581 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2641 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2701 agaacagtat ttggtatctg cgctctgctg aagccagtta cctcggaaaa agagttggta
     2761 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2821 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gattttctac cg
//
