LOCUS       sequence-110571-        2041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  construction of CYC1 promoter in HCKan-P receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-P-CYC1
FEATURES             Location/Qualifiers
     source          1..2041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      349..380
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        381..483
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             484..1299
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(557..576)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1323..1350
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1362..1949)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1442..1461)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1971..2000)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 ggctgagctc atttggcgag cgttggttgg tggatcaagc ccacgcgtag gcaatcctcg
       61 agcagatccg ccaggcgtgt atatatagcg tggatggcca ggcaacttta gtgctgacac
      121 atacaggcat atatatatgt gtgcgacgac acatgatcat atggcatgca tgtgctctgt
      181 atgtatataa aactcttgtt ttcttctttt ctctaaatat tctttcctta tacattagga
      241 cctttgcagc ataaattact atacttctat agacacgcaa acacaaatac acacactaag
      301 atgcgagacg actgaccatt taaatcatac ctgacctcca tagcagaaag tcaaaagcct
      361 ccgaccggag gcttttgact tgatcggcac gtaagaggtt ccaactttca ccataatgaa
      421 ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct
      481 aaaatgagcc atattcaacg ggaaacgtct tgctcgaggc cgcgattaaa ttccaacatg
      541 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca
      601 atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt
      661 agcgttgcca atgatgttac agatgagatg gtcaggctaa actggctgac ggaatttatg
      721 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact
      781 gcgatcccag ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat
      841 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt
      901 ccttttaacg gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt
      961 ttggttggtg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg
     1021 aaagaaatgc ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc
     1081 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga
     1141 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt
     1201 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat
     1261 aaattgcagt ttcacttgat gctcgatgag tttttctaat gagggcccaa atgtaatcac
     1321 ctggctcacc ttcgggtggg cctttctgcg ttgctggcgt ttttccatag gctccgcccc
     1381 cctgacgagc atcacaaaaa tcgatgctca agtcagaggt ggcgaaaccc gacaggacta
     1441 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1501 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1561 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1621 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1681 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1741 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1801 agaacagtat ttggtatctg cgctctgctg aagccagtta cctcggaaaa agagttggta
     1861 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1921 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gattttctac cgaagaaagg
     1981 cccacccgtg aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtccgtctc
     2041 g
//
