LOCUS       sequence-110574-        3224 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  systhesis of crtE gene in the beta-carotene pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: PMV-ycrtE
FEATURES             Location/Qualifiers
     source          1..3224
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     94..112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     121..138
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     122..138
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1327..1343)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1327..1343)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1355..1372)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1526..2114)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1606..1625)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2285..3076,3077..3145))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2908..2927
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(3146..3224,1..26))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
       61 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
      121 tgtaaaacga cggccagtcg tctcgatccg cagtgtcttg cgtctctatg gactacgcta
      181 acatcttgac cgctatccca ttggagttca ccccacaaga cgacatcgtt ttgttggaac
      241 cataccacta cttgggtaag aacccaggta aggaaatcag atctcaattg atcgaggctt
      301 tcaactactg gttggacgtt aagaaggaag acttggaagt tatccaaaac gttgttggta
      361 tgttgcacac cggttctttg ttgatggacg acgttgaaga ctcttctgtt ttgagaagag
      421 gttctccagt tgctcacttg atctacggta tcccacaaac catcaacacc gctaactacg
      481 tttacttctt ggcttaccaa gaaatcttca agctcagacc aaccccaatc ccaatgccag
      541 ttatcccacc atcttctgct tctttgcaat cttctgtttc ttctgcttct tcttcttctt
      601 ctgcttcttc tgaaaacggt ggaacctcta ccccaaactc tcaaatccca ttctctaagg
      661 acacctactt ggacaaggtt atcaccgacg aaatcttgtc tttgcacaga ggtcaaggtt
      721 tggaattgtt ctggagagac tctttgacct gtccatctga agaagaatac gttaagatgg
      781 ttttgggtaa gaccggtggt ttgttcagaa tcgctgttag attgatgatg gctaagtctg
      841 aatgtgacat cgacttcgtt caattggtta acttgatctc tatctacttc caaatccgcg
      901 acgactacat gaacttgcaa tcttctgaat acgctcacaa caagaacttc gctgaagact
      961 tgaccgaagg taagttctct ttcccaacca tccactctat ccacgctaac ccatctagta
     1021 gattggttat caacaccttg caaaagaagt ctacctctcc agaaatcttg caccactgtg
     1081 ttaactacat gagaaccgaa acccactctt tcgaatacac ccaagaagtt ttgaacacct
     1141 tgtctggtgc tttggaaaga gaattgggta gattgcaagg tgagttcgct gaagctaaca
     1201 gtagaatgga cttgggtgac gttgactctg aaggtagaac cggtaagaac gttaagttgg
     1261 aagctatctt gaagaagttg gctgacatcc cattgtaaag agacggagtc actgccaacc
     1321 gagacggtca tagctgtttc ctgtgtgccg cttcctcgct cactgactcg ctgcgctcgg
     1381 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttacccacag
     1441 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     1741 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1801 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1861 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1921 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     1981 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2041 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2101 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2161 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2221 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2281 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2341 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2401 gccccagtgc tgcaataata ccgcgggacc cacgctcacc ggctccagat ttatcagcaa
     2461 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2521 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2581 gcaacgttgt tgccatcgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2641 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2701 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gccgtgttat
     2761 cactcatggt tatggcagca ctacataatt ctcttactgt catgccatcc gtaagatgct
     2821 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2881 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2941 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3001 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3061 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3121 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3181 agggttattg tctcatgagc ggatacatat ttgaatgtat ttag
//
