LOCUS       sequence-110577-        2871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  construction of ycrtE gene in HCKan-O receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-O-ycrtE
FEATURES             Location/Qualifiers
     source          1..2871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1179..1210
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1211..1313
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1314..2129
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1387..1406)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2153..2180
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2192..2779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2272..2291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2801..2830)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gatggactac gctaacatct tgaccgctat cccattggag ttcaccccac aagacgacat
       61 cgttttgttg gaaccatacc actacttggg taagaaccca ggtaaggaaa tcagatctca
      121 attgatcgag gctttcaact actggttgga cgttaagaag gaagacttgg aagttatcca
      181 aaacgttgtt ggtatgttgc acaccggttc tttgttgatg gacgacgttg aagactcttc
      241 tgttttgaga agaggttctc cagttgctca cttgatctac ggtatcccac aaaccatcaa
      301 caccgctaac tacgtttact tcttggctta ccaagaaatc ttcaagctca gaccaacccc
      361 aatcccaatg ccagttatcc caccatcttc tgcttctttg caatcttctg tttcttctgc
      421 ttcttcttct tcttctgctt cttctgaaaa cggtggaacc tctaccccaa actctcaaat
      481 cccattctct aaggacacct acttggacaa ggttatcacc gacgaaatct tgtctttgca
      541 cagaggtcaa ggtttggaat tgttctggag agactctttg acctgtccat ctgaagaaga
      601 atacgttaag atggttttgg gtaagaccgg tggtttgttc agaatcgctg ttagattgat
      661 gatggctaag tctgaatgtg acatcgactt cgttcaattg gttaacttga tctctatcta
      721 cttccaaatc cgcgacgact acatgaactt gcaatcttct gaatacgctc acaacaagaa
      781 cttcgctgaa gacttgaccg aaggtaagtt ctctttccca accatccact ctatccacgc
      841 taacccatct agtagattgg ttatcaacac cttgcaaaag aagtctacct ctccagaaat
      901 cttgcaccac tgtgttaact acatgagaac cgaaacccac tctttcgaat acacccaaga
      961 agttttgaac accttgtctg gtgctttgga aagagaattg ggtagattgc aaggtgagtt
     1021 cgctgaagct aacagtagaa tggacttggg tgacgttgac tctgaaggta gaaccggtaa
     1081 gaacgttaag ttggaagcta tcttgaagaa gttggctgac atcccattgt agccgagacg
     1141 actgaccatt taaatcatac ctgacctcca tagcagaaag tcaaaagcct ccgaccggag
     1201 gcttttgact tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
     1261 taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatgagcc
     1321 atattcaacg ggaaacgtct tgctcgaggc cgcgattaaa ttccaacatg gatgctgatt
     1381 tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgat
     1441 tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca
     1501 atgatgttac agatgagatg gtcaggctaa actggctgac ggaatttatg cctcttccga
     1561 ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact gcgatcccag
     1621 ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg
     1681 cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaacg
     1741 gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttggtg
     1801 cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc
     1861 ataagctttt gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata
     1921 accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg
     1981 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
     2041 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
     2101 ttcacttgat gctcgatgag tttttctaat gagggcccaa atgtaatcac ctggctcacc
     2161 ttcgggtggg cctttctgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     2221 atcacaaaaa tcgatgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2641 ttggtatctg cgctctgctg aagccagtta cctcggaaaa agagttggta gctcttgatc
     2701 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2761 cagaaaaaaa ggatctcaag aagatccttt gattttctac cgaagaaagg cccacccgtg
     2821 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtccgtctc g
//
