LOCUS       sequence-110579-        3762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  construction of ycrtYB gene in HCKan-O receiving vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: HCKan-O-ycrtYB
FEATURES             Location/Qualifiers
     source          1..3762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      2070..2101
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        2102..2204
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2205..3020
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2278..2297)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      3044..3071
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(3083..3670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3163..3182)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3692..3721)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 gatgaccgct ttggcttact accaaatcca cttgatctac accttgccaa tcttgggttt
       61 gttgggtttg ttgacctctc caatcttgac caagttcgac atctacaaaa tctctatctt
      121 ggttttcatc gctttctctg ctaccacccc atgggactct tggatcatca gaaacggtgc
      181 ttggacctac ccatctgctg aatctggtca aggtgttttc ggaaccttct tggacgttcc
      241 atacgaagaa tacgctttct tcgttatcca aaccgttatc acaggtttgg tttacgtttt
      301 ggctaccaga cacttgttgc catctttggc tttgccaaag acccgatctt ctgctttgtc
      361 tttggctttg aaggctttga tcccattgcc aatcatctac ttgttcaccg ctcacccatc
      421 tccatctcca gacccattgg ttaccgacca ctacttctac atgagagctt tgtctttgtt
      481 gatcacccca ccaaccatgt tgttggctgc tttgtctggt gaatacgctt tcgactggaa
      541 gtctggtaga gctaagtcta ccatcgctgc tatcatgatc ccaaccgttt acttgatctg
      601 ggttgactac gttgctgttg gtcaagactc ttggtctatc aacgacgaaa agatcgttgg
      661 ttggagattg ggtggtgttt tgccaatcga agaagctatg ttcttcttgt tgaccaactt
      721 gatgatcgtt ttgggtttgt ctgcttgtga ccacacccag gctttgtact tgttgcacgg
      781 tagaaccatc tacggtaaca agaagatgcc atcttctttc ccattgatca ccccaccagt
      841 tttgtctttg ttcttctcta gtagaccata ctcttctcaa ccaaagagag acttggaatt
      901 ggctgttaag ttgttggaag aaaagtctcg atctttcttc gttgcttctg ctggtttccc
      961 atctgaagtt agagaaagat tggttggttt gtacgctttc tgtagagtta ccgacgactt
     1021 gatcgactct ccagaagttt cttctaaccc acacgctacc atcgacatgg tttctgactt
     1081 cttgaccttg ttgttcggtc caccattgca cccatctcaa ccagacaaaa tcttgtcttc
     1141 tccattgttg ccaccatctc acccaagtag accaacaggt atgtacccat tgccaccacc
     1201 accatctttg tctccagctg aattggttca attcttgacc gaaagagttc cagttcaata
     1261 ccacttcgct ttcagattgt tggctaagtt gcaaggtttg atcccaagat acccattgga
     1321 cgaattgttg agaggttaca ccaccgactt gatcttccca ttgtctaccg aagctgttca
     1381 agctagaaag accccaatcg aaaccaccgc tgacttgttg gactacggtt tgtgtgttgc
     1441 tggttctgtt gctgaattgt tggtttacgt ttcttgggct tctgctccat ctcaagttcc
     1501 agctaccatc gaagaaagag aagctgtttt ggttgctagt agagaaatgg gaaccgcttt
     1561 gcaattggtt aacatcgcta gagacatcaa gggtgacgct accgaaggta gattctactt
     1621 gccattgtct ttcttcggtt tgcgcgacga atctaagttg gctatcccaa ccgactggac
     1681 cgaaccaaga ccacaagact tcgacaagtt gttgtctttg tctccatctt ctaccttgcc
     1741 atcttctaac gcttctgaat ctttcagatt cgaatggaag acctactctt tgccattggt
     1801 tgcttacgct gaagacttgg ctaagcactc ttacaagggt atcgacagat tgccaaccga
     1861 agttcaagct ggtatgagag ctgcttgtgc ttcttacttg ttgatcggta gagaaatcaa
     1921 ggttgtttgg aagggtgacg ttggtgaaag aagaaccgtt gctggttgga gaagagttag
     1981 aaaggttttg tctgttgtta tgtctggttg ggaaggtcaa tagccgagac gactgaccat
     2041 ttaaatcata cctgacctcc atagcagaaa gtcaaaagcc tccgaccgga ggcttttgac
     2101 ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     2161 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatgagc catattcaac
     2221 gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
     2281 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga
     2341 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
     2401 cagatgagat ggtcaggcta aactggctga cggaatttat gcctcttccg accatcaagc
     2461 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatccca gggaaaacag
     2521 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
     2581 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac ggcgatcgcg
     2641 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttggt gcgagtgatt
     2701 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt
     2761 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
     2821 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     2881 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac
     2941 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcacttga
     3001 tgctcgatga gtttttctaa tgagggccca aatgtaatca cctggctcac cttcgggtgg
     3061 gcctttctgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3121 atcgatgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3181 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3241 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     3301 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3361 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3421 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3481 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     3541 gcgctctgct gaagccagtt acctcggaaa aagagttggt agctcttgat ccggcaaaca
     3601 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3661 aggatctcaa gaagatcctt tgattttcta ccgaagaaag gcccacccgt gaaggtgagc
     3721 cagtgagttg attgcagtcc agttacgctg gagtccgtct cg
//
