LOCUS       sequence-110583-        3172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  systhesis of vioD gene in the violacein pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, 
            Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J
  TITLE     YeastFab: the design and construction of standard biological parts 
            for metabolic engineering in Saccharomyces cerevisiae.
  JOURNAL   Nucleic Acids Res. 2015 May 8. pii: gkv464.
  PUBMED    25956650
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 May 8. pii: gkv464."
COMMENT     Sequence Label: pKMV-VioD
FEATURES             Location/Qualifiers
     source          1..3172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     50..68
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     77..94
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     78..94
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1276..1292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1276..1292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1304..1321)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1475..2063)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1555..1574)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2234..3049)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2234..3049)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2957..2976
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(3050..3154)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
       61 cacgaggccc tttcgttgta aaacgacggc cagtcgtctc gatccgcagt gtcttgcgtc
      121 tctgatgaaa atcttggtta tcggtgctgg tccagctggt ttggttttcg cttctcaatt
      181 gaagcaagct agaccattgt gggctatcga catcgttgaa aagaacgacg aacaagaagt
      241 tttgggttgg ggtgttgttt tgccaggtag accaggtcaa cacccagcta acccattgtc
      301 ttacttggac gctccagaaa gattgaaccc acaattcttg gaagacttca agttggttca
      361 ccacaacgaa ccatctttga tgtctaccgg tgttttgttg tgtggtgttg aaagaagagg
      421 tttggttcac gctttgagag acaagtgtcg atctcaaggt atcgctatca gattcgaatc
      481 tccattgttg gaacacggtg aattgccatt ggctgactac gacttggttg ttttggctaa
      541 cggtgttaac cacaagaccg ctcacttcac cgaggctttg gttccacaag ttgactacgg
      601 tagaaacaag tacatctggt acggaacctc tcaattgttc gaccaaatga acttggtttt
      661 cagaacccac ggtaaggaca tcttcatcgc tcacgcttac aagtactctg acaccatgtc
      721 taccttcatc gttgaatgtt ctgaagaaac ctacgctaga gctagattgg gtgaaatgtc
      781 tgaagaggct tctgctgaat acgttgctaa ggttttccaa gctgaattgg gtggtcacgg
      841 tttggtttct caaccaggtt tgggttggag aaacttcatg accttgtctc acgacagatg
      901 tcacgacggt aagttggttt tgttgggtga cgctttgcaa tctggtcact tctctatcgg
      961 tcacggaacc acaatggctg ttgttgttgc tcaattgttg gttaaggctt tgtgtaccga
     1021 agacggtgtt ccagctgctt tgaagagatt cgaagaaaga gctttgccat tggttcaatt
     1081 gttcagaggt cacgctgaca acagtagagt ttggttcgaa accgttgaag aaagaatgca
     1141 cttgtcttct gctgagttcg ttcaatcttt cgacgctaga agaaagtctt tgccaccaat
     1201 gccagaggct ttggctcaaa acttgagata cgctttgcaa agatagcaga gacggagtca
     1261 ctgccaaccg agacggtcat agctgtttcc tgtgtgccgc ttcctcgctc actgactcgc
     1321 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1381 tacccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1441 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1501 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1561 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1621 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1681 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1741 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1801 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     1861 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     1921 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     1981 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2041 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2101 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2161 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2221 cttggtctga cagttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     2281 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     2341 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     2401 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     2461 catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt
     2521 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     2581 tcattcgtga ttgcgcctga gcgagtcgaa atacgcgatc gctgttaaaa ggacaattac
     2641 aaacaggaat cgaatgcaac cggcgcagga acacggccag cgcatcaaca atattttcac
     2701 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gctgtggtga
     2761 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     2821 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     2881 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     2941 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     3001 aatttaatcg cggcctagag caagacgttt cccgttgaat atggctcata ctcttccttt
     3061 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3121 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gt
//
