LOCUS       sequence-110604-         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic
            loci using the Crispr/Cas technology. Can be converted by cassette 
            exchange into other Trojan exons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pT-GEM(2)
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    48..147
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
     protein_bind    154..187
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    194..328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(218..237)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     272..291
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(600..620)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          750..815
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 ggcattacct cctaagggaa cctccccact agtggtaccg gcgcgccccc aggtcagaag
       61 cggttttcgg gagtagtgcc ccaactgggg taacctttga gttctctcag ttgggggcgt
      121 agggtcgccg acatgacaca aggggttctc gagataactt cgtataatgt atgctatacg
      181 aagttatgct agcgatccag acatgataag atacattgat gagtttggac aaaccacaac
      241 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      301 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
      361 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
      421 ggctgattat gatctctagt caaggcacta tacatcaaat atttttccat aattttcttg
      481 tatagcagtg cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta
      541 ctaaacacag catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct
      601 acctttctct tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca
      661 ctagatggca tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc
      721 tcccattcat cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt
      781 ttaacacatt atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt
      841 agtttgtcca attatgtcac accacagaag taaggttcct tc
//
