LOCUS       sequence-110649-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recipient overexpressing vector to construct FLAG tagged fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pYC119
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     427..443
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     428..444
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(488..511)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(518..541)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(548..571)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(581..597)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(581..597)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gactcactat agggcgaatt gggtaccttt acctctatat cgtgttcgtg cgtatttctt
       61 cctctataat atgaaaagaa aggcagtagt cttgattttc tattcttcca aatattacaa
      121 gaatacccag aaccacaaga agcagaagaa actaactgct ttatatactt aatttcccgc
      181 cacaagctca tcgcccagaa gtttttttca gtttttcacc tgacaatctg gcagctcgct
      241 gaaaaatttt cacctggggt tgcctgctac gtaaagtgat cacgtttatg aatggcacat
      301 tatatagtgg cccagacggt ctgatggtag ttcatgtagt taagaataaa tactctgttt
      361 gtgtgatttc tatgtgtacg ttatatatat aaatattgtt gatggtggta gctgtgggtt
      421 gtgttctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag cccgggggat
      481 cttatcactt gtcatcgtca tctttgtagt cagccccttt atcgtcatcg tccttataat
      541 cggctccttt gtcatcgtca tctttgtagt ctgctcctag gatccactag ttctagaaaa
      601 cttagattag attgctatgc tttctttcta atgagcaaga agtaaaaaaa gttgtaatag
      661 aacaagaaaa atgaaactga aacttgagaa attgaagacc gtttattaac ttaa
//
