LOCUS       sequence-110687-         623 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Detect translation initiation from CUG start codon (E. coli lacZ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 623)
  AUTHORS   O'Connor M, Thomas CL, Zimmermann RA, Dahlberg AE
  TITLE     Decoding fidelity at the ribosomal A and P sites: influence of 
            mutations in three different regions of the decoding domain in 16S 
            rRNA.
  JOURNAL   Nucleic Acids Res. 1997 Mar 15;25(6):1185-93.
  PUBMED    9092628
REFERENCE   2  (bases 1 to 623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1997-03-15"; volume: "25(6)"; pages: "1185-93"
COMMENT     Sequence Label: pSG 413
FEATURES             Location/Qualifiers
     source          1..623
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     140..162
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     154..171
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     155..171
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(206..228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    217..233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(241..247,248..263,264..269))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 aaccaggcaa agcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
       61 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
      121 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt
      181 agcgtaatca gggtgaattc tgtttcctgt gtgaaattgt tatccgctca caattgtcga
      241 cattatacga gccgatgatt aattgtcaac acggccgacg cgctgggcta cgtcttgctg
      301 gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
      361 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
      421 cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcattgg accgctgatc
      481 gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc
      541 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc
      601 tcgacctgaa tggaagccac tgc
//
