LOCUS       sequence-110753-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bicistronic expression of mRFP-Utrophin and Neo resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  TITLE     Piel Lab Plasmids
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIRESneo3+mRFP-Utr_CH
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        30..48
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             103..777
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             103..777
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(233..251)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     501..520
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     695..718
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
       61 tggtaccgag ctcggatcga tatctgcggc ctagctagca ccatggcctc ctccgaggac
      121 gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa cggccacgag
      181 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      241 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc tcagttccag
      301 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
      361 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
      421 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
      481 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      541 accgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagat gaggctgaag
      601 ctgaaggacg gcggccacta cgacgccgag gtcaagacca cctacatggc caagaagccc
      661 gtgcagctgc ccggcgccta caagaccgac atcaagctgg acatcacctc ccacaacgag
      721 gactacacca tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcgcctcc
      781 ggaaccatgg ccaagtatgg agaacatgaa gccagtcctg acaatgggca gaacgaattc
      841 agtgatatca tt
//
