LOCUS       sequence-110778-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  wild type HIV-1 integrase in pET-15b.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Jenkins TM, Engelman A, Ghirlando R, Craigie R
  TITLE     A soluble active mutant of HIV-1 integrase: involvement of both the 
            core and carboxyl-terminal domains in multimerization.
  JOURNAL   J Biol Chem. 1996 Mar 29;271(13):7712-8.
  PUBMED    8631811
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1996-03-29"; volume: "271(13)"; pages: "7712-8"
COMMENT     Sequence Label: wt HIV-1 integrase in pET15b
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    62..86
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..123
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             142..159
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             169..186
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
       61 gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg
      121 agatatacca tgggcagcag ccatcatcat catcatcaca gcagcggcct ggtgccgcgc
      181 ggcagccata tgtttttaga tggaatagat aaggcccaag aagaacatga gaaatatcac
      241 agtaattgga gagcaatggc tagtgatttt aacctgccac ctgtagtagc aaaagaaata
      301 gtagccagct gtgataaatg tcagctaaaa ggggaagcca tgcatggaca agtagactgt
      361 agcccaggaa tatggcagct agattgtaca catttagaag gaaaagttat cttggtagca
      421 gttcatgtag ccagtggata tatagaagca gaagtaattc cagcagagac agggcaagaa
      481 acagcatact tcctcttaaa attagcagga agatggccag taaaaacagt acatacagac
      541 aatggcagca atttcaccag tactacagtt aaggccgcct gttggtgggc ggggatcaag
      601 caggaatttg gcattcccta caatccccaa agtcaggggg taatagaatc tatgaataaa
      661 gaattaaaga aaattatagg acaggtaaga gatcaggctg aacatcttaa gacagcagta
      721 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
      781 ggggaaagaa tagtagacat aatagcaaca gacatac
//
