LOCUS       sequence-110898-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  chondrocyte-specific COL11A2 promoter/enhancer lentiviral reporter 
            vector to select iChon cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Outani H, Okada M, Yamashita A, Nakagawa K, Yoshikawa H, Tsumaki N
  TITLE     Direct induction of chondrogenic cells from human dermal fibroblast 
            culture by defined factors.
  JOURNAL   PLoS One. 2013 Oct 16;8(10):e77365. doi: 
            10.1371/journal.pone.0077365. eCollection 2013.
  PUBMED    24146984
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-16"; volume: "8(10):e77365. doi"; pages: " 
            10.1371/journal.pone.0077365. eCollection 2013"
COMMENT     Sequence Label: pLe6-Delta -hLP-mcs/(EGFP-IresPuro-hInt)
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..80)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    133..157
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atccagaggt tgattgtcga tcattactaa ccggtacgcg tagaatcgag accgaggaga
       61 gggttaggga taggcttacc ttcgaaccgc gggccctcta gactcgagcg gccgccactg
      121 tgctggatat caaccacttt gtacaagaaa gctgggtcta gatatctcga catatgccga
      181 attcccaagc ttccagatct ctgggagagt ccatgattat caggagaagg gacatgccct
      241 caggagggca taaatagggg acatttggga tctagaactc agctttccag ggctcaaact
      301 ccctgcaagg gaaaggtcac ctcaccctca cttgcttctg aacagtacct gaatggatgg
      361 gaaatgcaaa ggtacctgga ggcagggcag catcagctgg cattcaaccc catgacactc
      421 ctgcccctgt ctctcctagc atctgcctct cttacgctct ctctttgtct tttagcttat
      481 gaatctgtct ctctctgtac tctctgaata cttctctcaa ctcttcatct gtctcctgtc
      541 tctctcactc tcttactctc tctgtctctt tatgttggtc tttctgtctc tgtctcttct
      601 gtcttcctcc atttctctca cattctgtcc atctttttct ctccctcgct ctcactctct
      661 ttccatatct ctcactctct gggtctctgg catctgtccc gtctccagca caaacaacat
      721 ctgggcaatc gatcatcctg gacacaggag gtgcaggggg gccacgagga agagatcaga
      781 gaagcagctc tatgagaggg gcttcaagca gctacagatc ccaggtttgg
//
