LOCUS       sequence-110944-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For phage display of antibody libraries by fusion to modified 
            geneVIII..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Barbas CF 3rd, Kang AS, Lerner RA, Benkovic SJ
  TITLE     Assembly of combinatorial antibody libraries on phage surfaces: the 
            gene III site.
  JOURNAL   Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7978-82.
  PUBMED    1896445
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1991-09-15"; volume: "88(18)"; pages: 
            "7978-82"
COMMENT     Sequence Label: pComb8
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     complement(38..103)
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     primer_bind     complement(341..357)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(342..358)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     sig_peptide     complement(372..437)
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     promoter        complement(497..515)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(536..552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(536..552)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(549..571)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    560..576
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(584..590,591..608,609..614))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    629..650
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcgattgcgg ccgcttaact ctagaactga cgagctcggc catggctggt tgggcagcga
       61 gtaataacaa tccagcggct gccgtaggca ataggtattt cattatgact gtctccttgg
      121 cgactagcta gtttagaatt ctatcagctt gctttcgagg tgaatttctt aaacagcttg
      181 ataccgatag ttgcgccgac aatgacaaca accatcgccc acgcataacc gatatattcg
      241 gtcgctgagg cttgcaggga gttaaaggcc gcttttgcgg gatcgtcacc ctcagcacta
      301 gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagc
      361 agtttcacct gggccatggc tggttgggca gcgagtaata acaatccagc ggctgccgta
      421 ggcaataggt atttcattat gactgtctcc ttgaaataga atttgcggcc gccaccgcgg
      481 tggccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat
      541 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      601 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      661 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      721 aacgcgc
//
