LOCUS       sequence-110945-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (OsU3 promoter), Bar resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pBUE411
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(636..683)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     RBS             763..771
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atcaattggc aagtcataaa atgcattaaa aaatattttc atactcaact acaaatccat
       61 gagtataact ataattataa agcaatgatt agaatctgac aaggattctg gaaaattaca
      121 taaaggaaag ttcataaatg tctaaaacac aagaggacat acttgtattc agtaacattt
      181 gcagcttttc taggtctgaa aatatatttg ttgcctagtg aataagcata atggtacaac
      241 tacaagtgtt ttactcctca tattaacttc ggtcattaga ggccacgatt tgacacattt
      301 ttactcaaaa caaaatgttt gcatatctct tataatttca aattcaacac acaacaaata
      361 agagaaaaaa caaataatat taatttgaga atgaacaaaa ggaccatatc attcattaac
      421 tcttctccat ccatttccat ttcacagttc gatagcgaaa accgaataaa aaacacagta
      481 aattacaagc acaacaaatg gtacaagaaa aacagttttc ccaatgccat aatactcaaa
      541 ctcagtagga ttctggtgtg tgcgcaatga aactgatgca ttgaacttga cgaacgttgt
      601 cgaaaccgat gatacgaacg aaagctctga gctcacttct tcttcttcgc ctgccccgcc
      661 ttcttcgtcg ccgctggccg cttgtcgccc ccgagctgag acaggtcgat gcgcgtctcg
      721 tagaggccgg taatcgactg gtggatgagg gtcgcgtcca ggacctcctt agtgcttgtg
      781 tacctcttgc gatcgatagt tgtgtcgaag tacttgaaag cagcagg
//
