LOCUS       sequence-110947-         689 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For phage display of antibody libraries by fusion to modified 
            geneVIII..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Barbas CF 3rd, Kang AS, Lerner RA, Benkovic SJ
  TITLE     Assembly of combinatorial antibody libraries on phage surfaces: the 
            gene III site.
  JOURNAL   Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7978-82.
  PUBMED    1896445
REFERENCE   2  (bases 1 to 689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1991-09-15"; volume: "88(18)"; pages: 
            "7978-82"
COMMENT     Sequence Label: pComb8
FEATURES             Location/Qualifiers
     source          1..689
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     52..117
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     primer_bind     131..147
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     132..148
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     sig_peptide     386..451
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     primer_bind     complement(512..531)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(513..531)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(538..555)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(538..554)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(547..569)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gctggccacc gcggtggcgg ccgcaaattc tatttcaagg agacagtcat aatgaaatac
       61 ctattgccta cggcagccgc tggattgtta ttactcgctg cccaaccagc catggcccag
      121 gtgaaactgc tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg
      181 ggatccacta gtgctgaggg tgacgatccc gcaaaagcgg cctttaactc cctgcaagcc
      241 tcagcgaccg aatatatcgg ttatgcgtgg gcgatggttg ttgtcattgt cggcgcaact
      301 atcggtatca agctgtttaa gaaattcacc tcgaaagcaa gctgatagaa ttctaaacta
      361 gctagtcgcc aaggagacag tcataatgaa atacctattg cctacggcag ccgctggatt
      421 gttattactc gctgcccaac cagccatggc cgagctcgtc agttctagag ttaagcggcc
      481 gcaatcgagg gggggcccgg tacccaattc gccctatagt gagtcgtatt acaattcact
      541 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      601 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      661 ttcccaacag ttgcgcagcc tgaatggcg
//
