LOCUS       sequence-110948-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For phage display of antibody libraries by fusion to modified 
            geneIII..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Barbas CF 3rd, Kang AS, Lerner RA, Benkovic SJ
  TITLE     Assembly of combinatorial antibody libraries on phage surfaces: the 
            gene III site.
  JOURNAL   Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7978-82.
  PUBMED    1896445
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1991-09-15"; volume: "88(18)"; pages: 
            "7978-82"
COMMENT     Sequence Label: pComb3
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     complement(38..103)
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     protein_bind    148..164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(172..178,179..196,197..202))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    217..238
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 tcgattgcgg ccgcttaact ctagaactga cgagctcggc catggctggt tgggcagcga
       61 gtaataacaa tccagcggct gccgtaggca ataggtattt cattatgact gtctccttgg
      121 cgactagcta gtttagaatt cgtgaaattg ttatccgctc acaattccac acaacatacg
      181 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      241 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgttact agctagcata
      301 ataacggaat acccaaaaga actggcatga ttaagactcc ttattacgca gtatgttagc
      361 aaacgtagaa aatacataca taaaggtggc aacatataaa agaaacgcaa agacaccacg
      421 gaataagttt attttgtcac aatcaataga aaattcatat ggtttaccag cgccaaagac
      481 aaaagggcga cattcaaccg attgagggag ggaaggtaaa tattgacgga aattattcat
      541 taaaggtgaa ttatcaccgt caccgacttg agccatttgg gaattagagc cagcaaaatc
      601 accagtagca ccattaccat tagcaaggcc ggaaacgtca ccaatgaaac catcgatagc
      661 agcaccgtaa tcagtagcga cagaatcaag tttgccttta gcgtcagact gtagcgcgtt
      721 ttcatcggca ttttcggtca tag
//
