LOCUS       sequence-110961-         878 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas based plant genome editing and gene regulation; expresses
            3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence
            (OsU3 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ
  TITLE     A CRISPR/Cas9 toolkit for multiplex genome editing in plants.
  JOURNAL   BMC Plant Biol. 2014 Nov 29;14(1):327.
  PUBMED    25432517
REFERENCE   2  (bases 1 to 878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol."; date: "2014-11-29"; volume: "14(1)"; pages: "327"
COMMENT     Sequence Label: pHUE411
FEATURES             Location/Qualifiers
     source          1..878
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    40..214
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 gtttttaatg tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga
       61 ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata caaatacata
      121 ctaagggttt cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt
      181 atctgggaac tactcacaca ttattatgga gaaactcgag cttgtcgatc gacagatccc
      241 ggtcggcatc tactctattt ctttgccctc ggacgagtgc tggggcgtcg gtttccacta
      301 tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt
      361 gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa
      421 gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg
      481 agcatatacg cccggagtcg tggcgatcct gcaagctccg gatgcctccg ctcgaagtag
      541 cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg
      601 tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg
      661 tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag
      721 tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg
      781 tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc
      841 catgtagtgt attgaccgat tccttgcggt ccgaatgg
//
