LOCUS       sequence-111041-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Centromeric E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG217
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..43
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     141..157
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     142..158
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(192..208)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(192..208)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(316..335)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(317..335)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(342..359)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(342..358)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(351..373)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        475..714
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
ORIGIN
        1 tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctgggtac
       61 aagtgacaat atatatatat atatataata acgacatcgt tatctgtaaa tacggccgaa
      121 gctgggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct
      181 gcagcccggg ggatccacta gttctagagc caccgcggtg gagctccaat tcgccctata
      241 ggcggccgcg aaaagaaata tagtcttcaa ggttatatta tacaggttta tatattattc
      301 tgtttctcca attcgcccta tagtgagtcg tattacaatt cactggccgt cgttttacaa
      361 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccgatc
      421 gagaggcatc gcacagtttt agcgaggaaa actcttcaat agttttgcca gcggaattgc
      481 acttgcaatt acataaaaaa ttccggcggt ttttcgcgtg tgactcaatg tcgaaatacc
      541 tgcctaatga acatgaacat cgcccaaatg tatttgaaga cccgctggga gaagttcaag
      601 atatataagt aacaagcagc caatagtata aaaaaaaatc tgagtttatt acctttcctg
      661 gaatttcagt gaaaaactgc taattataga gacatatcac agagttactc actaatgact
      721 aacgaaaagg tctggataga gaagttggat aatccaactc tttcagtgtt accacatgac
      781 tttttacgcc cacaacaaga accttatacg aaacaagcta catattcgtt acagctacct
      841 cagctcgatg tgcctcatga tagtttttct aacaaatacg ctgtcgcttt gagtgtatgg
      901 gctgcattga tatatagagt aactggtgac gatgatattg ttctttatat tgcgaataac
      961 aaaatcttaa gattcaatat tcaaccaacg tggtcattta atgagctgta ttctacaatt
     1021 aacaatgagt tgaacaagct caattctatt gaggccaatt tttcctttga cgagctagct
     1081 gaaaaa
//
