LOCUS       sequence-111063-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Horman SR, Janas MM, Litterst C, Wang B, MacRae IJ, Sever MJ, 
            Morrissey DV, Graves P, Luo B, Umesalma S, Qi HH, Miraglia LJ, 
            Novina CD, Orth AP
  TITLE     Akt-mediated phosphorylation of argonaute 2 downregulates cleavage 
            and upregulates translational repression of MicroRNA targets.
  JOURNAL   Mol Cell. 2013 May 9;50(3):356-67. doi: 
            10.1016/j.molcel.2013.03.015. Epub 2013 Apr 18.
  PUBMED    23603119
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.03.015"; journalName: "Mol Cell"; date: 
            "2013-05-9- 9"; volume: "50"; issue: "3"; pages: "356-67"
COMMENT     Sequence Label: pDEST26 Ago2 S387E
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             115..132
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    142..166
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
       61 gggaccgatc cagcctccgg actctagcct aggccgcgga ccatggcgta ctaccatcac
      121 catcaccatc actctagatc aacaagtttg tacaaaaaag caggcttaat gtactcggga
      181 gccggccccg cacttgcacc tcctgcgccg ccgcccccca tccaaggata tgccttcaag
      241 cctccaccta gacccgactt tgggacctcc gggagaacaa tcaaattaca ggccaatttc
      301 ttcgaaatgg acatccccaa aattgacatc tatcattatg aattggatat caagccagag
      361 aagtgcccga ggagagttaa cagggaaatc gtggaacaca tggtccagca ctttaaaaca
      421 cagatctttg gggatcggaa gcccgtgttt gacggcagga agaatctata cacagccatg
      481 ccccttccga ttgggaggga caaggtggag ctggaggtca cgctgccagg agaaggcaag
      541 gatcgcatct tcaaggtgtc catcaagtgg gtgtcctgcg tgagcttgca ggcgttacac
      601 gatgcacttt cagggcggct gcccagcgtc ccttttgaga cgatccaggc cctggacgtg
      661 gtcatgaggc acttgccatc catgaggtac acccccgtgg gccgctcctt cttcaccgcg
      721 tccgaaggct gctctaaccc tcttggcggg ggccgagaag tgtggtttgg cttccatcag
      781 tccgtccggc cttctctctg gaaaatgatg ctgaatattg atgtgtcagc aacagcgttt
      841 tacaaggcac agccagtaat cgagtttgtt tgtgaagttt tggattttaa aagtattgaa
      901 gaacaacaaa aacctctgac agattcccaa agggtaaagt ttac
//
