LOCUS       sequence-111109-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Sp dCas9-GFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pHAGE-TO-dCas9-GFP
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(67..780)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             complement(793..813)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atccagaggt tgattatcta gattacccta ctcgaggctc ccatcgatgc cagagcggcc
       61 gccagatttg tacagttcat ccataccatg cgtgatgccc gctgcggtta cgaactccag
      121 cagaaccata tgatcgcgtt tctcgttcgg atctttagac agaacgcttt gcgtgctcag
      181 atagtgattg tctggcagca gaacaggacc atcaccgatt ggagtgtttt gctggtagtg
      241 atcagccagc tgcacgctgc catcctccac gttgtggcga attttaaaat tcgctttaat
      301 gccatttttt tgtttatcgg cggtgatgta aacattgtgg ctgttaaaat tgtattccag
      361 cttatggccc aggatattgc cgtcttcttt aaagtcaatg cctttcagct caatgcggtt
      421 taccagggta tcgccttcaa atttcacttc cgcacgcgtt ttgtacgtgc cgtcatcctt
      481 aaaggaaatc gtgcgttcct gcacatagcc ttccggcatg gcggacttga agaagtcatg
      541 ctgcttcata tggtccggat aacgagcaaa gcactgaaca ccataagtca gcgtcgttac
      601 cagagtcggc caaggtaccg gcagtttacc agtagtacag atgaacttca gcgtcagttt
      661 accattagtt gcgtcacctt caccctcgcc acgcacggaa aacttatgac cgttgacatc
      721 accatccagt tccaccagaa tagggacgac accagtgaac agctcttcgc ctttacgcat
      781 acttccggat cctaccttcc tcttcttctt gggagagcca ctagtagagc cgtctccacc
      841 gagctgagag aggtcgattc ttgtttcata gagccccgta attgactgat gaatcag
//
