LOCUS       sequence-111158-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to label reporter mRNAs. Lentiviral expression of the PP7 coat 
            protein fused to EGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Halstead JM, Lionnet T, Wilbertz JH, Wippich F, Ephrussi A, Singer 
            RH, Chao JA
  TITLE     Translation. An RNA biosensor for imaging the first round of 
            translation from single cells to living animals.
  JOURNAL   Science. 2015 Mar 20;347(6228):1367-671. doi: 
            10.1126/science.aaa3380.
  PUBMED    25792328
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-03-20- 20"; volume: "347"; 
            issue: "6228"; pages: "1367-671"
COMMENT     Sequence Label: phage ubc nls ha pcp gfp
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      58..67
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             70..90
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             94..120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             94..120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             121..132
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      523..532
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(574..595)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggcggcc
       61 gccatgggcc caaaaaagaa aagaaaagtt ggctacccct acgacgtgcc cgactacgcc
      121 atcgaaggcc gccatatgct agcctccaaa accatcgttc tttcggtcgg cgaggctact
      181 cgcactctga ctgagatcca gtccaccgca gaccgtcaga tcttcgaaga gaaggtcggg
      241 cctctggtgg gtcggctgcg cctcacggct tcgctccgtc aaaacggagc caagaccgcg
      301 tatcgcgtca acctaaaact ggatcaggcg gacgtcgttg attccggact tccgaaagtg
      361 cgctacactc aggtatggtc gcacgacgtg acaatcgttg cgaatagcac cgaggcctcg
      421 cgcaaatcgt tgtacgattt gaccaagtcc ctcgtcgcga cctcgcaggt cgaagatctt
      481 gtcgtcaacc ttgtgccgct gggccgtgcg gatccaccgg tcgccaccat ggtgagcaag
      541 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      601 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      661 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      721 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      781 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggac
//
