LOCUS       sequence-111249-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recipient overexpressing vector to construct YFP tagged fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pYC25
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        36..434
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     438..454
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     438..454
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(488..516)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 attaaccctc actaaaggga acaaaagctg gagctcatag cttcaaaatg tttctactcc
       61 ttttttactc ttccagattt tctcggactc cgcgcatcgc cgtaccactt caaaacaccc
      121 aagcacagca tactaaattt cccctctttc ttcctctagg gtgtcgttaa ttacccgtac
      181 taaaggtttg gaaaagaaaa aagagaccgc ctcgtttctt tttcttcgtc gaaaaaggca
      241 ataaaaattt ttatcacgtt tctttttctt gaaaattttt tttttgattt ttttctcttt
      301 cgatgacctc ccattgatat ttaagttaat aaacggtctt caatttctca agtttcagtt
      361 tcatttttct tgttctatta caactttttt tacttcttgc tcattagaaa gaaagcatag
      421 caatctaatc taagttttct agaactagtg gatcctaatg agtaaaggag aagaactttt
      481 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
      541 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aattgatttg
      601 cactactgga aaactacctg ttccatggcc aacacttgtc actactttgg gttatggtct
      661 aatgtgcttt gctagatacc cagatcatat gaaacggcat gactttttca agagtgccat
      721 gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac
      781 acgtgctgaa gtcaagtttg aaggtgatac cc
//
