LOCUS       sequence-111270-         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of eGFP in T. cruzi. The NheI-linearized form of this 
            plasmid could be efficiently integrated in to T. cruzi genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Peng D, Kurup SP, Yao PY, Minning TA, Tarleton RL
  TITLE     CRISPR-Cas9-Mediated Single-Gene and Gene Family Disruption in 
            Trypanosoma cruzi.
  JOURNAL   MBio. 2014 Dec 30;6(1). pii: e02097-14. doi: 10.1128/mBio.02097-14.
  PUBMED    25550322
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2014-12-30"; volume: "6(1). pii: e02097-14. doi"; pages: " 
            10.1128/mBio.02097-14"
COMMENT     Sequence Label: pTrex-n-eGFP
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
       61 cttcttgacg agttcttctg aggggatcga tccgtgcaca ggcgttagcc actctcagga
      121 ttgccagcaa gtcttctgaa tttgatcttt ggatgctcgg gcagctcaaa gaggaatctt
      181 agtcacaacc tgccgaagga gcgccgcttc ttcaagattg tgtcgtggta cgacaacgag
      241 tggggatact cccaccgcgt ggtggacctt gtacgccaca tggcctcgaa ggatcgttcg
      301 gcaaggttgt aggcgtggcg atgacttcag gtctttcttt tgcgaatagg gatcttataa
      361 tacacgatgc gtgtcccgtg atgatcgtta ccggtgctgc cacgatccaa gtgacacagt
      421 gtcaagagca aaacaatttt acttttccct ttaaggacaa caacaaaaaa atatataact
      481 tttttttctt tttttttttt gaaattatat ttatggtcat ctttgggaaa caaaaagcag
      541 caatttaatg atgcggaagg atgagtgaaa taatgtttaa tcaatgtacg aggatttggg
      601 gtattgcaag gaaaatgtag atgatttgat tgggtgtatg atgcagcttg tggtaatttt
      661 ttacaacttt gcgttattgg aagattttgg ttgttcagtt tctttttgtt tataactgac
      721 tgttttaatt tagtgtgatt tttttttttt aaggatttaa gctgtttttc tttttttttc
      781 tttttcttca ctgatggtgt ttaaggtgta gtttagtgtt tgtgcattat gcgtc
//
