LOCUS       sequence-111298-         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
  TITLE     Linear DNA for rapid prototyping of synthetic biological circuits in
            an Escherichia coli based TX-TL cell-free system.
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. 
            Epub 2013 Dec 4.
  PUBMED    24303785
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400131a";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "387-97"
COMMENT     Sequence Label: pBADmod1-linker2-gamS
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             357..374
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(427..444)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      597..683
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 actctctact gtttctccat acccgttttt taactttaag aaggagatat accatgggaa
       61 acgcttatta cattcaggat cgtcttgagg ctcagagctg ggcgcgtcac taccagcagc
      121 tcgcccgtga agagaaagag gcagaactgg cagacgacat ggaaaaaggc ctgccccagc
      181 acctgtttga atcgctatgc atcgatcatt tgcaacgcca cggggccagc aaaaaatcca
      241 ttacccgtgc gtttgatgac gatgttgagt ttcaggagcg catggcagaa cacatccggt
      301 acatggttga aaccattgct caccaccagg ttgatattga ttcagaggta ctcgagcacc
      361 accaccacca ccactgaatt aaggtctcca gcttggctgt tttggcggat gagagaagat
      421 tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc
      481 tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg
      541 tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa
      601 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      661 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacg
//
