LOCUS       sequence-111380-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses sgRNA under U6c promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pU6c:sgRNA#4
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..21
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5..21
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgttgtaaaa cgacggccag tcttaagctc gggcccggtc tctccaatcc gagagtctgt
       61 gaatgtttcg atgggccgtt tgctggcgtc tgagcatgtt gtcctctggt ctgcggcggg
      121 ggaaggtccg cgcatgcctg tgggggaagg cgaaggacac gaggacaaaa gatcagttac
      181 gtcacagaag aactcatcca atcacacaag cctaaaacca gcttcctctg catatgcttt
      241 acagttcaaa aatactacgg taaacctcta caaaactgct ggtttcaaac tttgaaggtt
      301 ttaagcgttt gcaggtttgc cccgaagagg tttactgtca tgtttgaggt aaatgagttg
      361 aataagtagg tttatccact taccacatgg cagaaacata cccagaagtc ccgggtttat
      421 atagcagttc tccaggctcc tagttcgaga gacgacactg cagactgtcg accgtctctg
      481 tttaagagct atgctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact
      541 tgaaaaagtg gcaccgagtc ggtgcttttt ttaaacctga gagacctcta gaccagccag
      601 gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa
      661 cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
      721 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaa
//
