LOCUS       sequence-111381-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses sgRNA under U6a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pU6a:sgRNA#2
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..26
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10..26
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cacgacgttg taaaacgacg gccagtctta agctcgggcc cggtctctag tcaagtctct
       61 cagcgttttc tccagcctcg gtcattcagt aagacgagtt tcctgctctc ggcgtctttt
      121 gttctggtct tcaaggaggg gggaaatgtt ctgcgcatgc ctgtggggga gggagaagga
      181 cacgtcactg aaaacgtccc tgcatcacac cgagacaccc aatcactcaa gccaagacca
      241 gataattttg catatgcttt acagtttgaa aattaccacg gtaatccctc acacaaactc
      301 tggatttgag atccttcagg ttttatcagt ttgcaggttt atgtcaccat gatatagggt
      361 cagacttgat ctaagggagc tgaataagtg gtttagtcac tcaccacctc ccaaaaacat
      421 acccagaagt ccctggtata tatagctctc cctccagctc ttggttcgag agacgacact
      481 gcagactgtc gaccgtctct gtttaagagc tatgctggaa acagcatagc aagtttaaat
      541 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttaaactca
      601 agagacctct agaccagcca ggacagaaat gcctcgactt cgctgctacc caaggttgcc
      661 gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata agcctgttcg
      721 gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
      781 gaacgcagcg gtggtaacgg cgcagtgg
//
