LOCUS       sequence-111383-         706 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses codon optimized Cas9 in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pME-Cas9
FEATURES             Location/Qualifiers
     source          1..706
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..21)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             116..124
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 cacctttctc ttcttcttag gagatctcac cggtgagtcg ccacccagct gagacaggtc
       61 aatccttgtt tcgtacaggc cggtaatact ctgatggatc agtgtagcgt ccagcacctc
      121 cttagtggag gtgtacctct ttctatcgat tgtagtgtcg aagtacttga aagctgcagg
      181 tgcgcccagg tttgtcagag tgaacagatg aatgatattt tcggcctgct ccctgatggg
      241 cttatctctg tgtttgttgt aagcagacag gaccttatcc agatttgcgt cggccaggat
      301 cactctttta gagaactcgc taatctgttc aatgatctcg tccagataat gcttgtgctg
      361 ctccacaaac agctgtttct gttcgttatc ctctggagat cctttcagct tctcgtagtg
      421 gcttgccaga tacaggaagt tcacgtactt agaaggcagg gccagttcat tgcctttctg
      481 cagctctcca gcgcttgcca gcatgcgttt cctgccgttt tccagctcaa acagactgta
      541 cttgggcagt ttaatgatca ggtctttctt cacttcctta taacctttgg cctccagaaa
      601 atcgattggg ttcttctcga aagagcttct ctccataatt gtgataccca gcagctcctt
      661 gacagatttc agtttcttgc ttttgccctt ctccacctta gcgacc
//
