LOCUS       sequence-111426-        9796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET biosensor Destination vector for yeast expression. Encodes a 
            FRET pair for fusion with ligand binding domain of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, 
            Frommer WB
  TITLE     Abscisic acid dynamics in roots detected with genetically encoded 
            FRET sensors.
  JOURNAL   eLife. 2014 Apr 15;3:e01741. doi: 10.7554/eLife.01741.
  PUBMED    24737862
REFERENCE   2  (bases 1 to 9796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-04-15"; volume: "3:e01741. doi"; pages: " 
            10.7554/eLife.01741"
COMMENT     Sequence Label: pDRFLIP38
FEATURES             Location/Qualifiers
     source          1..9796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             465..487
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             507..524
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             528..560
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(564..572,573..587)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(603..605,606..1316)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITLGMDELYK"
     protein_bind    1329..1453
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1478..1483,1484..1501,1502..1508)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(1628..1647)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2621..2926
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2805..2824
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2967..3091)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(3105..3107,3108..3818)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALFKDPNEKRDHMVLLE
                     FLTAAGITLGMDELYK"
     CDS             3819..3848
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3886..3902)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3887..3903)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3927..3946)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3928..3946)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      4094..4281
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4149..4173)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4293..4310)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4293..4309)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4302..4324)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4745..6091
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(6202..7005)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7006..7221)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(7246..7264)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7332..7436
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7437..7505,7506..8297)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7655..7674)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8468..9056
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8957..8976
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9210..9227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9344..9365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9380..9385,9386..9403,9404..9410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9418..9434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9423..9445
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9442..9458
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9442..9458
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtagaactat gagctttttt tgtttctgtt ttcctttttt ttttttttac ctctgtggaa
       61 attgttactc tcacactctt tagttcgttt gtttgttttg tttattccaa ttatgaccgg
      121 tgacgaaacg tggtcgatgg tgggtaccgc ttatgctccc ctccattagt ttcgattata
      181 taaaaaggcc aaatattgta ttattttcaa atgtcctatc attatcgtct aacatctaat
      241 ttctcttaaa ttttttctct ttctttccta taacaccaat agtgaaaatc tttttttctt
      301 ctatatctac aaaaactttt tttttctatc aacctcgttg ataaattttt tctttaacaa
      361 tcgttaataa ttaattaatt ggaaaataac cattttttct ctcttttata cacacattca
      421 aaagaaagaa aaaaaatata ccccagcctc gatctagaaa taattttgtt taactttaag
      481 aaggagatat acatatgcgg ggttctcatc atcatcatca tcatggtatg gctagcatga
      541 ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat ccgggcctcg
      601 aggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      661 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      721 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      781 tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc
      841 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
      901 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
      961 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     1021 agcttgagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     1081 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     1141 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     1201 acctgagcta ccagtccgcc ctgttcaaag accccaacga gaagcgcgat cacatggtcc
     1261 tgctggagtt cctgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggta
     1321 ccgatatcac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
     1381 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
     1441 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc
     1501 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
     1561 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     1621 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     1681 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     1741 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     1801 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     1861 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     1921 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1981 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     2041 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     2101 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atgggcttcc
     2161 atgtcggcag aatgcttaat gaattacaca gtactgcgat gagtggcagg gcggggcgta
     2221 aacgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     2281 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
     2341 atgaagcagc gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat
     2401 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     2461 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
     2521 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
     2581 acctataaaa cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     2641 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     2701 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     2761 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     2821 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     2881 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     2941 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt
     3001 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     3061 tttctcgttc agctttcttg tacaaagtgg tgatatcgac tagtgtgagc aagggcgagg
     3121 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     3181 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     3241 tcatctgcac caccggtaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     3301 ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3361 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     3421 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     3481 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca
     3541 tcagcgacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
     3601 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     3661 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     3721 ccctgttcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcctgaccg
     3781 ccgccgggat cactctcggc atggacgagc tgtacaagga acaaaaattg ataagtgagg
     3841 aagatttata agggccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct
     3901 cgaggggggg cccggtaccc aattcgccct atagtgagtc gtattacgcg cggatccagc
     3961 tttggacttc ttcgccagag gtttggtcaa gtctccaatc aaggttgtcg gcttgtctac
     4021 cttgccagaa atttacgaaa agatggaaaa gggtcaaatc gttggtagat acgttgttga
     4081 cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa
     4141 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgar aattcttatt
     4201 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg aggtcgctct
     4261 tattgaccac acctctaccg gcatgccaat tcactggccg tcgttttaca acgtcgtgac
     4321 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     4381 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4441 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     4501 ataatcggat cgtacttgtt acccatcatt gaattttgaa catccgaacc tgggagtttt
     4561 ccctgaaaca gatagtatat ttgaacctgt ataataatat atagtctagc gctttacgga
     4621 agacaatgta tgtatttcgg ttcctggaga aactattgca tctattgcat aggtaatctt
     4681 gcacgtcgca tccccggttc attttctgcg tttccatctt gcacttcaat agcatatctt
     4741 tgttaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat
     4801 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct
     4861 attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg
     4921 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga
     4981 gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga
     5041 gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat
     5101 aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact
     5161 ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt
     5221 actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac
     5281 cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg
     5341 tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt
     5401 tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc
     5461 taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc
     5521 aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa
     5581 gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac
     5641 agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa
     5701 gcgctctgaa gttcctatac tttctagcta gagaatagga acttcggaat aggaacttca
     5761 aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca
     5821 ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg
     5881 gcatagtgcg tgtttatgct taaatgcgta cttatatgcg tctatttatg taggatgaaa
     5941 ggtagtctag tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt
     6001 cagcactacc ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct
     6061 tcaatgctat catttccttt gatattggat cgatccgatg ataagctgtc aaacatgaga
     6121 attgggtaat aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc
     6181 atttacttat aatacagttt tttagttttg ctggccgcat cttctcaaat atgcttccca
     6241 gcctgctttt ctgtaacgtt caccctctac cttagcatcc cttccctttg caaatagtcc
     6301 tcttccaaca ataataatgt cagatcctgt agagaccaca tcatccacgg ttctatactg
     6361 ttgacccaat gcgtctccct tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc
     6421 gtaaccttca tctcttccac ccatgtctct ttgagcaata aagccgataa caaaatcttt
     6481 gtcgctcttc gcaatgtcaa cagtaccctt agtatattct ccagtagata gggagccctt
     6541 gcatgacaat tctgctaaca tcaaaaggcc tctaggttcc tttgttactt cttctgccgc
     6601 ctgcttcaaa ccgctaacaa tacctgggcc caccacaccg tgtgcattcg taatgtctgc
     6661 ccattctgct attctgtata cacccgcaga gtactgcaat ttgactgtat taccaatgtc
     6721 agcaaatttt ctgtcttcga agagtaaaaa attgtacttg gcggataatg cctttagcgg
     6781 cttaactgtg ccctccatgg aaaaatcagt caagatatcc acatgtgttt ttagtaaaca
     6841 aattttggga cctaatgctt caactaactc cagtaattcc ttggtggtac gaacatccaa
     6901 tgaagcacac aagtttgttt gcttttcgtg catgatatta aatagcttgg cagcaacagg
     6961 actaggatga gtagcagcac gttccttata tgtagctttc gacatgattt atcttcgttt
     7021 cctgcatgtt tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc
     7081 aacactacat atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt
     7141 ctgttcggag attaccgaat caaaaaaatt tcaaggaaac cgaaatcaaa aaaaagaata
     7201 aaaaaaaaat gatgaattga aaagctaatt cttgaagacg aaagggcctc gtgatacgcc
     7261 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     7321 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7381 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7441 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7501 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7561 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     7621 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     7681 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     7741 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     7801 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     7861 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     7921 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     7981 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8041 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8101 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8161 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8221 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8281 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8341 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8401 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8461 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8521 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     8581 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     8641 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     8701 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     8761 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     8821 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     8881 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     8941 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9001 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9061 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     9301 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     9361 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     9421 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttcctg
     9481 aaacggagaa acataaacag gcattgctgg gatcacccat acatcactct gttttgcctg
     9541 accttttccg gtaatttgaa aacaaacccg gtctcgaagc ggagatccgg cgataattac
     9601 cgcagaaata aacccataca cgagacgtag aaccagccgc acatggccgg agaaactcct
     9661 gcgagaattt cgtaaactcg cgcgcattgc atctgtattt cctaatgcgg cacttccagg
     9721 cctcgatcga gaccgtttat ccattgcttt tttgttgtct ttttccctcg ttcacagaaa
     9781 gtctgaagaa gctata
//
