LOCUS       sequence-111429-        9769 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET biosensor Destination vector for yeast expression. Encodes a 
            FRET pair for fusion with ligand binding domain of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, 
            Frommer WB
  TITLE     Abscisic acid dynamics in roots detected with genetically encoded 
            FRET sensors.
  JOURNAL   eLife. 2014 Apr 15;3:e01741. doi: 10.7554/eLife.01741.
  PUBMED    24737862
REFERENCE   2  (bases 1 to 9769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-04-15"; volume: "3:e01741. doi"; pages: " 
            10.7554/eLife.01741"
COMMENT     Sequence Label: pDRFLIP43
FEATURES             Location/Qualifiers
     source          1..9769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             465..487
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             507..524
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             528..560
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(564..572,573..587)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    1302..1426
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1451..1456,1457..1474,1475..1481)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(1601..1620)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2594..2899
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2778..2797
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2940..3064)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(3078..3080,3081..3791)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALFKDPNEKRDHMVLLE
                     FLTAAGITLGMDELYK"
     primer_bind     complement(3120..3141)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3381..3400)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             3792..3821
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(3859..3875)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3860..3876)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3900..3919)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3901..3919)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      4067..4254
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(4122..4146)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(4266..4283)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4266..4282)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4275..4297)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4718..6064
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(6175..6978)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6979..7194)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(7219..7237)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7305..7409
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7410..7478,7479..8270)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7628..7647)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8441..9029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8930..8949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9183..9200
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9317..9338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9353..9358,9359..9376,9377..9383)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9391..9407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9396..9418
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9415..9431
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9415..9431
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtagaactat gagctttttt tgtttctgtt ttcctttttt ttttttttac ctctgtggaa
       61 attgttactc tcacactctt tagttcgttt gtttgttttg tttattccaa ttatgaccgg
      121 tgacgaaacg tggtcgatgg tgggtaccgc ttatgctccc ctccattagt ttcgattata
      181 taaaaaggcc aaatattgta ttattttcaa atgtcctatc attatcgtct aacatctaat
      241 ttctcttaaa ttttttctct ttctttccta taacaccaat agtgaaaatc tttttttctt
      301 ctatatctac aaaaactttt tttttctatc aacctcgttg ataaattttt tctttaacaa
      361 tcgttaataa ttaattaatt ggaaaataac cattttttct ctcttttata cacacattca
      421 aaagaaagaa aaaaaatata ccccagcctc gatctagaaa taattttgtt taactttaag
      481 aaggagatat acatatgcgg ggttctcatc atcatcatca tcatggtatg gctagcatga
      541 ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat ccgggcctcg
      601 aggtttctaa aggtgaagaa ttgtttacgg gcgtcgtccc gatcctcgtg gaactcgacg
      661 gggatgttaa cgggcataag ttttcggtca gcggggaagg ggagggggac gcgacgtatg
      721 ggaagctcac tctcaagctg atctgtacga cggggaaact cccggtcccg tggccgacgc
      781 tggtcacgac gctgggatac gggctccaat gctttgcgag gtatccggac cacatgaaac
      841 agcatgactt tttcaaatcg gcgatgccgg agggatacgt gcaggaacgg acgatctttt
      901 tcaaagacga tgggaactat aagacgcggg cggaagtcaa gtttgaaggg gacacgctcg
      961 tcaaccggat cgaactcaag gggattgact tcaaagagga tgggaacata ctcggccata
     1021 agctcgaata caattacaac tcgcataacg tatacatcac cgcggataag caaaagaacg
     1081 ggatcaaagc caatttcaaa atccggcata acatagagga tgggggggtc caactggcgg
     1141 atcactatca gcaaaacacg ccgatagggg atgggccggt cctcctcccg gataaccatt
     1201 acctctcgta ccaaagcgcg ctcttcaagg acccgaatga gaaacgggac cacatggttc
     1261 tcctggagtt cctcacggcg gcgggcatag gtaccgatat cacaagtttg tacaaaaaag
     1321 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
     1381 aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg
     1441 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     1501 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1561 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1621 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1681 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1741 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1801 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     1861 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1921 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1981 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     2041 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     2101 tcaggttcat catgccgttt gtgatgggct tccatgtcgg cagaatgctt aatgaattac
     2161 acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaagc
     2221 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     2281 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     2341 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     2401 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     2461 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     2521 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaacttttgc tgacgagaac
     2581 aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
     2641 gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct
     2701 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
     2761 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat
     2821 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
     2881 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc
     2941 atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct
     3001 aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag
     3061 tggtgatatc gactagtgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     3121 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     3181 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggt aagctgcccg
     3241 tgccctggcc caccctcgtg accaccctga cctggggcgt gcagtgcttc gcccgctacc
     3301 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     3361 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     3421 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     3481 acatcctggg gcacaagctg gagtacaacg ccatcagcga caacgtctat atcaccgccg
     3541 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggca
     3601 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     3661 tgcccgacaa ccactacctg agcacccagt ccgccctgtt caaagacccc aacgagaagc
     3721 gcgatcacat ggtcctgctg gagttcctga ccgccgccgg gatcactctc ggcatggacg
     3781 agctgtacaa ggaacaaaaa ttgataagtg aggaagattt ataagggccg ggctgcagga
     3841 attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta cccaattcgc
     3901 cctatagtga gtcgtattac gcgcggatcc agctttggac ttcttcgcca gaggtttggt
     3961 caagtctcca atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga
     4021 aaagggtcaa atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg
     4081 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
     4141 tgactcttag gttttaaaac garaattctt attcttgagt aactctttcc tgtaggtcag
     4201 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc
     4261 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     4321 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     4381 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt
     4441 ctccttacgc atctgtgcgg tatttcacac cgcataatcg gatcgtactt gttacccatc
     4501 attgaatttt gaacatccga acctgggagt tttccctgaa acagatagta tatttgaacc
     4561 tgtataataa tatatagtct agcgctttac ggaagacaat gtatgtattt cggttcctgg
     4621 agaaactatt gcatctattg cataggtaat cttgcacgtc gcatccccgg ttcattttct
     4681 gcgtttccat cttgcacttc aatagcatat ctttgttaac gaagcatctg tgcttcattt
     4741 tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
     4801 ttttacagaa cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc
     4861 atttttgtaa aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc
     4921 tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat
     4981 acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct
     5041 tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca
     5101 ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa
     5161 aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt
     5221 caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa
     5281 cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta
     5341 ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac
     5401 tctatgaata gttcttacta caattttttt gtctaaagag taatactaga gataaacata
     5461 aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt
     5521 atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga
     5581 agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa
     5641 agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag
     5701 ctagagaata ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg
     5761 aaaatgcaac gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt
     5821 gttgcctgta tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc
     5881 gtacttatat gcgtctattt atgtaggatg aaaggtagtc tagtacctcc tgtgatatta
     5941 tcccattcca tgcggggtat cgtatgcttc cttcagcact accctttagc tgttctatat
     6001 gctgccactc ctcaattgga ttagtctcat ccttcaatgc tatcatttcc tttgatattg
     6061 gatcgatccg atgataagct gtcaaacatg agaattgggt aataactgat ataattaaat
     6121 tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt
     6181 ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc
     6241 taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc
     6301 tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc
     6361 taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc
     6421 tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc
     6481 cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag
     6541 gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg
     6601 gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc
     6661 agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa
     6721 aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc
     6781 agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa
     6841 ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc
     6901 gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt
     6961 atatgtagct ttcgacatga tttatcttcg tttcctgcat gtttttgttc tgtgcagttg
     7021 ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa
     7081 tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa
     7141 atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaaaagcta
     7201 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     7261 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7321 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7381 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7441 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7501 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7561 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7621 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7681 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7741 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7801 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7861 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7921 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7981 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8041 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8101 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8161 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8221 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8281 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8341 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8401 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8461 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8521 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8581 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8641 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8701 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8761 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8821 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8881 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8941 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9001 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9061 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9121 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9181 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     9241 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     9301 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     9361 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     9421 acagctatga ccatgattac gccaagcttc ctgaaacgga gaaacataaa caggcattgc
     9481 tgggatcacc catacatcac tctgttttgc ctgacctttt ccggtaattt gaaaacaaac
     9541 ccggtctcga agcggagatc cggcgataat taccgcagaa ataaacccat acacgagacg
     9601 tagaaccagc cgcacatggc cggagaaact cctgcgagaa tttcgtaaac tcgcgcgcat
     9661 tgcatctgta tttcctaatg cggcacttcc aggcctcgat cgagaccgtt tatccattgc
     9721 ttttttgttg tctttttccc tcgttcacag aaagtctgaa gaagctata
//
