LOCUS       sequence-111469-         645 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses sgRNA(tyr) under U6a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Yin L, Maddison LA, Li M, Kara N, LaFave MC, Varshney GK, Burgess 
            SM, Patton JG, Chen W
  TITLE     Multiplex Conditional Mutagenesis Using Transgenic Expression of 
            Cas9 and sgRNAs.
  JOURNAL   Genetics. 2015 Apr 8. pii: genetics.115.176917.
  PUBMED    25855067
REFERENCE   2  (bases 1 to 645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Apr 8. pii: genetics.115.176917."
COMMENT     Sequence Label: pU6a:sgRNA(tyr)
FEATURES             Location/Qualifiers
     source          1..645
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(72..147)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(608..625)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(608..624)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(617..639)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cggcaacctt gggtagcagc gaagtcgagg catttctgtc ctggctggtc tagaggtctc
       61 tgactaaaaa agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt
      121 ttaacttgct atttctagct ctaaaacccc cagaagtcct ccagtccgaa ccaagagctg
      181 gagggagagc tatatatacc agggacttct gggtatgttt ttgggaggtg gtgagtgact
      241 aaaccactta ttcagctccc ttagatcaag tctgacccta tatcatggtg acataaacct
      301 gcaaactgat aaaacctgaa ggatctcaaa tccagagttt gtgtgaggga ttaccgtggt
      361 aattttcaaa ctgtaaagca tatgcaaaat tatctggtct tggcttgagt gattgggtgt
      421 ctcggtgtga tgcagggacg ttttcagtga cgtgtccttc tccctccccc acaggcatgc
      481 gcagaacatt tcccccctcc ttgaagacca gaacaaaaga cgccgagagc aggaaactcg
      541 tcttactgaa tgaccgaggc tggagaaacg ctgagagact tactgagaga ccgggcccga
      601 gcttaagact ggccgtcgtt ttacaacgtc gtgactggga aaaca
//
