LOCUS       sequence-111474-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses destabilized Citrine in yeast..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: FRP1432
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             141..857
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MRSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
ORIGIN
        1 ttcttaaact tcttaaattc tacttttata gttagtcttt tttttagttt taaaacacca
       61 agaacttagt ttcgaataaa cacacataaa caaacaaaac tagtggatcc cccgggctgc
      121 aggaattcga tatcaagctt atgagatcta aaggtgaaga attattcact ggtgttgtcc
      181 caattttggt tgaattagat ggtgatgtta atggtcacaa attttctgtc tccggtgaag
      241 gtgaaggtga tgctacttac ggtaaattga ccttaaaatt tatttgtact actggtaaat
      301 tgccagttcc atggccaacc ttagtcacta ctttaggtta tggtttgatg tgttttgcta
      361 gatacccaga tcatatgaaa caacatgact ttttcaagtc tgccatgcca gaaggttatg
      421 ttcaagaaag aactattttt ttcaaagatg acggtaacta caagaccaga gctgaagtca
      481 agtttgaagg tgatacctta gttaatagaa tcgaattaaa aggtattgat tttaaagaag
      541 atggtaacat tttaggtcac aaattggaat acaactataa ctctcacaat gtttacatca
      601 tggctgacaa acaaaagaat ggtatcaaag ttaacttcaa aattagacac aacattgaag
      661 atggttctgt tcaattagct gaccattatc aacaaaatac tccaattggt gatggtccag
      721 tcttgttacc agacaaccat tacttatcct atcaatctaa gttatccaaa gatccaaacg
      781 aaaagagaga ccacatggtc ttgttagaat ttgttactgc tgctggtatt acccatggta
      841 tggatgaatt gtacaaagga tccacttggt ctactgttag aagatccggt ttggaaactc
      901 aattcagagc tttggacttc ttcgccagag gtttggtcaa gtctccaatc aaggttgtcg
      961 gcttgtctac cttgccagaa atttacgaaa agatggaaaa g
//
