LOCUS       sequence-111477-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  yeast expression vector p414GPD modified such that it is Gateway 
            converted and introduces a 3xFLAG tag at the N-terminus of the 
            insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: p414GPD-3xFLAG-GW
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     66..82
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     66..82
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             99..164
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    165..289
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(314..319,320..337,338..344)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(464..483)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 aattctactt ttatagttag tctttttttt agttttaaaa caccaagaac ttagtttcga
       61 cggattctag aactagtgga tcccccgggc tgcagatgga ctacaaagac catgacggtg
      121 attataaaga tcatgatatc gattacaagg atgacgatga caagacaagt ttgtacaaaa
      181 aagctgaacg agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata
      241 aaaaacagac tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt
      301 aggcacccca ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta
      361 ggatccgtcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
      421 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
      481 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
      541 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
      601 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
      661 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
      721 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
      781 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
      841 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
      901 ccccgttttc
//
