LOCUS       sequence-111479-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  yeast expression vector p414GPD modified such that it is Gateway 
            converted and introduces a 3xFLAG tag at the N-terminus of the 
            insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: p414GPD-3xFLAG-GW
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(3..192)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     204..222
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     protein_bind    241..365
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(406..711)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(508..527)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gccttcgagc gtcccaaaac cttctcaagc aaggttttca gtataatgtt acatgcgtac
       61 acgcgtctgt acagaaaaaa aagaaaaatt tgaaatataa ataacgttct taatactaac
      121 ataactataa aaaaataaat agggacctag acttcaggtt gtctaactcc ttccttttcg
      181 gttagagcgg atgtgggggg agggcgtgaa tgtaagcgtg acataactaa ttacatgact
      241 accactttgt acaagaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
      301 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      361 ctatggtcga cctgcagact ggctgtgtat aagggagcct gacatttata ttccccagaa
      421 catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc
      481 cccgataacg gaaaccggca cactggccat atcggtggtc atcatgcgcc agctttcatc
      541 cccgatatgc accaccgggt aaagttcacg ggagacttta tctgacagca gacgtgcact
      601 ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata atatcactct gtacatccac
      661 aaacagacga taacggctct ctcttttata ggtgtaaacc ttaaactgca tttcaccagc
      721 ccctgttctc gtcagcaaaa gagccgttca tttcaataaa ccgggcgacc tcagccatcc
      781 cttcctgatt ttccgctttc cagcgttcgg cacgcagacg acgggcttca ttctgcatgg
      841 ttgtgcttac cagaccggag atattgacat catatatgcc ttgagcaact gatagctgtc
      901 gctgtcaact gtcactgtaa tacgctgctt catagcatac ctctttttga catacttcgg
      961 gtatacatat cagtatatat tcttat
//
