LOCUS       sequence-111510-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes g1 gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pRPR1_g1gRNA_RPR1t
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(353..415)
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     misc_RNA        complement(428..503)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 agtagtggaa caaaagcaaa tgaaggattt catgcgtttg tactctaatc tggtagaaag
       61 atgtttcaca gactgtgtca atgacttcac aacatcaaag ctaaccaata aggaacaaac
      121 atgcatcatg aagtgctcag aaaagttctt gaagcatagc gaacgtgtag ggcagcgttt
      181 ccaagaacaa aacgctgcct tgggacaagg cttgggccga taaggtgtac tggcgtatat
      241 atatctaatt atgtatctct ggtgtagccc atttttagca tgtaaatata aagagaaacc
      301 atatctaatc taaccaaatc caaacaaaat tcaatagtta ctatcgcttt tttctttctg
      361 tatcgcaaat aagtgaaaat taaaaaagaa agattaaatt ggaagttgga tatggctcga
      421 gaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa
      481 cttgctattt ctagctctaa aacaaaatgt tgaaagtatt agtaagcttc tgccaatcgc
      541 agctcccaga gtttcgttcc cagccgcgag caccacagcg taccatgtgc gccacgaggc
      601 ctcaaacgta aaacaatcga aacgaaaaga aacagactat aggggagtat agagacagcc
      661 ggccaataag aagaggaaaa agaaatacta gcgtttatca atgtgggtcg ttaacatatc
      721 ctgttgacaa tgattacagg ttaaaaggta gcgtaagtga atattaacta tggatattct
      781 tatacttaga taagagacat ataaaacaca cgatgattga ttgattttta tgaccaatat
      841 atgtaattcg taattcagat
//
