LOCUS       sequence-111511-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes c8 gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pRPR1_c8gRNA_RPR1t
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(341..403)
                     /label=RPR1 terminator
                     /note="transcription terminator for the S. cerevisiae RNase
                     P RNA gene"
     misc_RNA        complement(416..491)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aaagcaaatg aaggatttca tgcgtttgta ctctaatctg gtagaaagat gtttcacaga
       61 ctgtgtcaat gacttcacaa catcaaagct aaccaataag gaacaaacat gcatcatgaa
      121 gtgctcagaa aagttcttga agcatagcga acgtgtaggg cagcgtttcc aagaacaaaa
      181 cgctgccttg ggacaaggct tgggccgata aggtgtactg gcgtatatat atctaattat
      241 gtatctctgg tgtagcccat ttttagcatg taaatataaa gagaaaccat atctaatcta
      301 accaaatcca aacaaaattc aatagttact atcgcttttt tctttctgta tcgcaaataa
      361 gtgaaaatta aaaaagaaag attaaattgg aagttggata tggctcgaga aaaaagcacc
      421 gactcggtgc cactttttca agttgataac ggactagcct tattttaact tgctatttct
      481 agctctaaaa cgggtactgt atgtacatac aaagcttctg ccaatcgcag ctcccagagt
      541 ttcgttccca gccgcgagca ccacagcgta ccatgtgcgc cacgaggcct caaacgtaaa
      601 acaatcgaaa cgaaaagaaa cagactatag gggagtatag agacagccgg ccaataagaa
      661 gaggaaaaag aaatactagc gtttatcaat gtgggtcgtt aacatatcct gttgacaatg
      721 attacaggtt aaaaggtagc gtaagtgaat attaactatg gatattctta tacttagata
      781 agagacatat aaaacacacg atgattgatt gatttttatg accaatatat gtaattcgta
      841 att
//
